Zhang Enjiao, Xu Zhongfei, Duan Weiyi, Huang Shaohui, Lu Li
Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, PR China.
Department of Oromaxillofacial-Head and Neck Surgery, School of Stomatology, China Medical University, Shenyang, PR China.
PLoS One. 2017 Jun 13;12(6):e0176044. doi: 10.1371/journal.pone.0176044. eCollection 2017.
MicroRNAs play important roles in the development of human cancers. This case-control study is to evaluate the roles of the polymorphisms in pre-miRNAs on risk of oral cancer in a Chinese population.
The genotypes of three polymorphisms were determined in 340 patients with oral squamous cell cancer and 340 healthy controls who were frequency matched for age and sex. Odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated to assess the association. All analyses were performed using the SPSS software. 3.154() 0.001.
For miR-499 rs3746444, individuals carrying homozygous CC genotype had increased risks of oral cancer compared with the homozygous wild TT genotype (adjusted OR was 3.154, 95%CI was 1.555-6.397, P value was 0.001). The C allele of miR-499 rs3746444 was associated with a higher risk of oral cancer with significant odds ratio of 1.453. In the stratified analyses by sex, the associations between miR-499 rs3746444 and miR-146a rs2910164 polymorphisms with the susceptibility of oral squamous cell cancer were significant in males. However, with 1/4 as many subjects there were no significant associations between the three polymorphisms and oral cancer risks in females. The joint effects of miRNA polymorphisms and smoking on the risk of OSCC were analyzed and the results suggested that the association between microRNA genetic variants and OSCC risk was modified by smoking.
These findings suggest that miR-499 rs3746444 and miR-146a rs2910164 polymorphisms may contribute to genetic susceptibility to oral squamous cell cancer.
微小RNA在人类癌症发展中发挥重要作用。本病例对照研究旨在评估前体微小RNA中的多态性在中国人群口腔癌风险中的作用。
在340例口腔鳞状细胞癌患者和340例年龄和性别频率匹配的健康对照中确定三种多态性的基因型。计算优势比（OR）和95%置信区间（95%CI）以评估关联性。所有分析均使用SPSS软件进行。3.154（）0.001。
对于miR-499 rs3746444，与纯合野生型TT基因型相比，携带纯合CC基因型的个体患口腔癌的风险增加（调整后的OR为3.154，95%CI为1.555 - 6.397，P值为0.001）。miR-499 rs3746444的C等位基因与口腔癌风险较高相关，优势比为1.453。在按性别分层分析中，miR-499 rs3746444和miR-146a rs2910164多态性与口腔鳞状细胞癌易感性之间的关联在男性中显著。然而，女性受试者数量仅为男性的四分之一，三种多态性与女性口腔癌风险之间无显著关联。分析了微小RNA多态性与吸烟对口腔鳞状细胞癌风险的联合作用，结果表明吸烟改变了微小RNA基因变异与口腔鳞状细胞癌风险之间的关联。
这些发现表明，miR-499 rs3746444和miR-146a rs2910164多态性可能导致口腔鳞状细胞癌的遗传易感性。
