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法国和瑞典有机与传统养猪生产中的:患病率与抗菌药物耐药性

in Organic and Conventional Pig Production in France and Sweden: Prevalence and Antimicrobial Resistance.

作者信息

Kempf Isabelle, Kerouanton Annaelle, Bougeard Stéphanie, Nagard Bérengère, Rose Valérie, Mourand Gwénaëlle, Osterberg Julia, Denis Martine, Bengtsson Björn O

机构信息

French Agency for Food, Environmental and Occupational Health and Safety (Anses), Mycoplasmology - Bacteriology Unit, Bretagne Loire UniversityPloufragan, France.

French Agency for Food, Environmental and Occupational Health and Safety (Anses), Hygiene and Quality of Poultry and Pig Products Unit, Bretagne Loire UniversityPloufragan, France.

出版信息

Front Microbiol. 2017 May 29;8:955. doi: 10.3389/fmicb.2017.00955. eCollection 2017.

Abstract

The purpose of the study was to evaluate and compare the prevalence and antimicrobial resistance of in conventional and organic pigs from France and Sweden. Fecal or colon samples were collected at farms or at slaughterhouses and cultured for . The minimum inhibitory concentrations of ciprofloxacin, nalidixic acid, streptomycin, tetracycline, erythromycin, and gentamicin were determined by microdilution for a total of 263 French strains from 114 pigs from 50 different farms and 82 Swedish strains from 144 pigs from 54 different farms. Erythromycin resistant isolates were examined for presence of the emerging rRNA methylase (B) gene. The study showed that within the colon samples obtained in each country there was no significant difference in prevalence of between pigs in organic and conventional productions [France: conventional: 43/58 (74%); organic: 43/56 (77%) and Sweden: conventional: 24/36 (67%); organic: 20/36 (56%)]. In France, but not in Sweden, significant differences of percentages of resistant isolates were associated with production type (tetracycline, erythromycin) and the number of resistances was significantly higher for isolates from conventional pigs. In Sweden, the number of resistances of fecal isolates was significantly higher compared to colon isolates. The (B) gene was not detected in the 87 erythromycin resistant strains tested.

摘要

本研究的目的是评估和比较法国和瑞典传统养殖与有机养殖猪的[某种细菌]流行率及抗菌药物耐药性。在养殖场或屠宰场采集粪便或结肠样本,并对[某种细菌]进行培养。采用微量稀释法测定了来自法国50个不同养殖场114头猪的263株菌株以及来自瑞典54个不同养殖场144头猪的82株菌株对环丙沙星、萘啶酸、链霉素、四环素、红霉素和庆大霉素的最低抑菌浓度。对红霉素耐药菌株检测新兴的rRNA甲基化酶(B)基因的存在情况。研究表明,在每个国家采集的结肠样本中,有机养殖和传统养殖猪的[某种细菌]流行率无显著差异[法国:传统养殖:43/58(74%);有机养殖:43/56(77%);瑞典:传统养殖:24/36(67%);有机养殖:20/36(56%)]。在法国,而非瑞典,耐药菌株百分比的显著差异与养殖类型有关(四环素、红霉素),且传统养殖猪分离株的耐药数量显著更高。在瑞典,粪便分离株的耐药数量相比结肠分离株显著更高。在所检测的87株红霉素耐药菌株中未检测到(B)基因。

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