环状RNA hsa_circ_0003159在胃癌中的表达下调及其临床意义
Reduced expression of circRNA hsa_circ_0003159 in gastric cancer and its clinical significance.
作者信息
Tian Mengqian, Chen Ruoyu, Li Tianwen, Xiao Bingxiu
机构信息
Zhejiang Key Laboratory of Pathophysiology, Department of Biochemistry and Molecular Biology, Medical School of Ningbo University, Ningbo, China.
出版信息
J Clin Lab Anal. 2018 Mar;32(3). doi: 10.1002/jcla.22281. Epub 2017 Jun 15.
BACKGROUND
Circular RNAs (circRNAs) play a crucial role in the occurrence of several diseases including cancers. However, little is known about circRNAs' diagnostic values for gastric cancer, one of the worldwide most common diseases of mortality.
METHODS
The hsa_circ_0003159 levels in 108 paired gastric cancer tissues and adjacent non-tumorous tissues from surgical patients with gastric cancer were first detected by real-time quantitative reverse transcription-polymerase chain reaction. Then, the relationships between hsa_circ_0003159 expression levels in gastric cancer tissues and the clinicopathological factors of patients with gastric cancer were analyzed. Finally, its diagnostic value was evaluated through the receiver operating characteristic curve.
RESULTS
Compared with paired adjacent non-tumorous tissues, hsa_circ_0003159 expression was significantly down-regulated in gastric cancer tissues. What is more, we found that hsa_circ_0003159 expression levels were significantly negatively associated with gender, distal metastasis, and tumor-node-metastasis stage.
CONCLUSIONS
All of the results suggest that hsa_circ_0003159 may be a potential cancer marker of patients with gastric cancer.
背景
环状RNA（circRNAs）在包括癌症在内的多种疾病的发生中起着关键作用。然而，关于circRNAs对胃癌（全球最常见的致死性疾病之一）的诊断价值，人们所知甚少。
方法
首先通过实时定量逆转录-聚合酶链反应检测108例胃癌手术患者的配对胃癌组织和癌旁非肿瘤组织中hsa_circ_0003159的水平。然后，分析胃癌组织中hsa_circ_0003159表达水平与胃癌患者临床病理因素之间的关系。最后，通过受试者工作特征曲线评估其诊断价值。
结果
与配对的癌旁非肿瘤组织相比，hsa_circ_0003159在胃癌组织中的表达明显下调。此外，我们发现hsa_circ_0003159表达水平与性别、远处转移和肿瘤-淋巴结-转移分期显著负相关。
结论
所有结果表明，hsa_circ_0003159可能是胃癌患者的一种潜在癌症标志物。
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