Daszkowska-Golec Agata, Skubacz Anna, Marzec Marek, Slota Michal, Kurowska Marzena, Gajecka Monika, Gajewska Patrycja, Płociniczak Tomasz, Sitko Krzysztof, Pacak Andrzej, Szweykowska-Kulinska Zofia, Szarejko Iwona
Department of Genetics, Faculty of Biology and Environmental Protection, University of Silesia in KatowiceKatowice, Poland.
Department of Microbiology, Faculty of Biology and Environmental Protection, University of Silesia in KatowiceKatowice, Poland.
Front Plant Sci. 2017 Jun 2;8:942. doi: 10.3389/fpls.2017.00942. eCollection 2017.
() encodes a small subunit of the cap-binding complex (CBC), which is involved in the conserved cell processes related to RNA metabolism in plants and, simultaneously, engaged in the signaling network of drought response, which is dependent on ABA. Here, we report the enhanced tolerance to drought stress of barley mutant in the gene manifested at the morphological, physiological, and transcriptomic levels. Physiological analyses revealed differences between the mutant and its WT in response to a water deficiency. The mutant exhibited a higher relative water content (RWC), a lower stomatal conductance and changed epidermal pattern compared to the WT after drought stress. Transcriptome analysis using the Agilent Barley Microarray integrated with observed phenotypic traits allowed to conclude that the mutant exhibited better fitness to stress conditions by its much more efficient and earlier activation of stress-preventing mechanisms. The network hubs involved in the adjustment of mutant to the drought conditions were proposed. These results enabled to make a significant progress in understanding the role of CBP20 in the drought stress response.
（）编码帽结合复合体（CBC）的一个小亚基，该复合体参与植物中与RNA代谢相关的保守细胞过程，同时参与依赖脱落酸的干旱响应信号网络。在此，我们报道了大麦突变体在基因水平上对干旱胁迫的耐受性增强，这在形态、生理和转录组水平上均有体现。生理分析揭示了突变体与其野生型在应对水分亏缺时的差异。干旱胁迫后，与野生型相比，突变体表现出更高的相对含水量（RWC）、更低的气孔导度以及改变的表皮模式。使用安捷伦大麦微阵列结合观察到的表型性状进行转录组分析，可以得出结论，突变体通过更高效、更早地激活应激预防机制，对胁迫条件表现出更好的适应性。提出了参与突变体适应干旱条件调节的网络枢纽。这些结果使得在理解CBP20在干旱胁迫响应中的作用方面取得了重大进展。
