Lima Natália da Silva, Numata Erica de Paula, Mesquita Leonardo Mendes de Souza, Dias Pollyana Hammoud, Vilegas Wagner, Gambero Alessandra, Ribeiro Marcelo Lima
Laboratory of Immunopharmacology and Molecular Biology, Clinical Pharmacology and Gastroenterology Unit, Sao Francisco University Medical School, Braganca Paulista-SP 12916-900, Brazil.
Laboratory of Bioprospection and Natural products (LBPN), UNESP-São Paulo State University/Coastal Campus of Sao Vicente, Pça Infante Dom Henrique S/N, São Vicente, São Paulo 11330-900, Brazil.
Nutrients. 2017 Jun 20;9(6):635. doi: 10.3390/nu9060635.
Guarana () is a plant originated in Brazil that presents a beneficial effect on body weight control and metabolic alterations. The aim of this study was to evaluate the effects of guarana on genes and miRNAs related to adipogenesis in 3T3L1 cells. The anti-adipogenic effect of guarana was evaluated by Oil Red-O staining. Gene and miRNA expression levels were determined by real time PCR. The Cebpα and β-catenin nuclear translocation were evaluated using immunocytochemistry. Our data indicated that the triglyceride-reducing effect of guarana was dose-dependent from 100 to 300 µg/mL (-12%, -20%, -24% and -40%, respectively, < 0.0001). An up-regulation of the anti-adipogenic genes , , , and and a down-regulation of pro-adipogenic genes , and were also observed. Furthermore, guarana repressed mmu-miR-27b-3p, mmu-miR-34b-5p and mmu-miR-760-5p, that contributed for up-regulation of their molecular targets , and . Additionally, cells treated with guarana presented an increase on β-catenin nuclear translocation ( < 0.0018). In summary, our data indicate that guarana has an anti-adipogenic potential due to its ability to modulate miRNAs and genes related to this process. Together our data demonstrate the important role of guarana as a putative therapeutic agent.
瓜拉那（）是一种原产于巴西的植物，对体重控制和代谢改变具有有益作用。本研究的目的是评估瓜拉那对3T3L1细胞中与脂肪生成相关的基因和微小RNA（miRNA）的影响。通过油红O染色评估瓜拉那的抗脂肪生成作用。通过实时聚合酶链反应（PCR）测定基因和miRNA的表达水平。使用免疫细胞化学评估Cebpα和β-连环蛋白的核转位。我们的数据表明，瓜拉那降低甘油三酯的作用在100至300μg/mL范围内呈剂量依赖性（分别为-12%、-20%、-24%和-40%，<0.0001）。还观察到抗脂肪生成基因、、、和的上调以及促脂肪生成基因、和的下调。此外，瓜拉那抑制了mmu-miR-27b-3p、mmu-miR-34b-5p和mmu-miR-760-5p，这导致它们的分子靶标、和上调。此外，用瓜拉那处理的细胞β-连环蛋白核转位增加（<0.0018）。总之，我们的数据表明瓜拉那具有抗脂肪生成潜力，因为它能够调节与此过程相关的miRNA和基因。我们的数据共同证明了瓜拉那作为一种潜在治疗剂的重要作用。
