Chung Brian Y, Ro Jennifer, Hutter Sabine A, Miller Kylie M, Guduguntla Lakshmi S, Kondo Shu, Pletcher Scott D
Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USA.
Program in Cellular and Molecular Biology, University of Michigan, Ann Arbor, MI 48109, USA.
Cell Rep. 2017 Jun 20;19(12):2441-2450. doi: 10.1016/j.celrep.2017.05.085.
Proper regulation of sleep-wake behavior and feeding is essential for organismal health and survival. While previous studies have isolated discrete neural loci and substrates important for either sleep or feeding, how the brain is organized to coordinate both processes with respect to one another remains poorly understood. Here, we provide evidence that the Drosophila Neuropeptide F (NPF) network forms a critical component of both adult sleep and feeding regulation. Activation of NPF signaling in the brain promotes wakefulness and adult feeding, likely through its cognate receptor NPFR. Flies carrying a loss-of-function NPF allele do not suppress sleep following prolonged starvation conditions, suggesting that NPF acts as a hunger signal to keep the animal awake. NPF-expressing cells, specifically those expressing the circadian photoreceptor cryptochrome, are largely responsible for changes to sleep behavior caused by NPF neuron activation, but not feeding, demonstrating that different NPF neurons separately drive wakefulness and hunger.
正确调节睡眠-觉醒行为和进食对于机体健康和生存至关重要。虽然先前的研究已经分离出对睡眠或进食重要的离散神经位点和底物，但大脑如何组织以相互协调这两个过程仍知之甚少。在这里，我们提供证据表明果蝇神经肽F（NPF）网络是成体睡眠和进食调节的关键组成部分。大脑中NPF信号的激活可能通过其同源受体NPFR促进觉醒和成年果蝇进食。携带功能丧失性NPF等位基因的果蝇在长期饥饿条件下不会抑制睡眠，这表明NPF作为一种饥饿信号使动物保持清醒。表达NPF的细胞，特别是那些表达昼夜节律光感受器隐花色素的细胞，在很大程度上负责由NPF神经元激活引起的睡眠行为变化，但不负责进食行为变化，这表明不同的NPF神经元分别驱动觉醒和饥饿。
