Kara Murat, Yolbaş Servet, Şahin Cem, Koca Süleyman Serdar
Department of Medical Genetics, Muğla Sıtkı Koçman University School of Medicine, Muğla, Turkey.
Department of Rheumatology, Fırat University School of Medicine, Elazığ, Turkey.
Eur J Rheumatol. 2017 Jun;4(2):83-86. doi: 10.5152/eurjrheum.2017.16056. Epub 2017 Apr 11.
Sirtuins (SIRTs) play a prominent role in metabolism, apoptosis, aging, inflammation, and epigenetics. Inflammation, apoptosis, and epigenetics are pathogenic issues in rheumatoid arthritis (RA). This study aimed to evaluate SIRT2 and SIRT3 mRNA expressions in patients with RA.
Fifty-four patients with RA and 26 healthy controls were enrolled. Disease activity was determined using the disease activity score (DAS) 28-erythrocyte sedimentation rate (ESR) (score of >2.6 was considered to be active). SIRT2 and SIRT3 mRNA expressions in the extracellular plasma were investigated by real-time PCR.
SIRT3 mRNA expression was higher in the RA group than in the healthy control group (4.64 fold, p<0.001), whereas SIRT2 mRNA expression was relatively lower in the RA group than in the healthy control group (0.55 fold, p=0.109). However, SIRT2 (1.73 fold, p=0.065) and SIRT3 (3.58 fold, p=0.051) mRNA expressions were relatively higher in patients with active RA than in those with inactive RA.
In RA, SIRT3 mRNA expression is increased, whereas SIRT2 mRNA expression is decreased. Conversely, SIRT2 and SIRT3 mRNA expressions increase in active RA. Therefore, the fate of each SIRT may differ in RA.
沉默调节蛋白（SIRTs）在代谢、细胞凋亡、衰老、炎症和表观遗传学中发挥着重要作用。炎症、细胞凋亡和表观遗传学是类风湿关节炎（RA）的致病因素。本研究旨在评估RA患者中SIRT2和SIRT3 mRNA的表达。
招募了54例RA患者和26例健康对照者。使用疾病活动评分（DAS）28-红细胞沉降率（ESR）来确定疾病活动度（评分>2.6被认为是活动期）。通过实时PCR研究细胞外血浆中SIRT2和SIRT3 mRNA的表达。
RA组中SIRT3 mRNA表达高于健康对照组（4.64倍，p<0.001），而RA组中SIRT2 mRNA表达相对低于健康对照组（0.55倍，p=0.109）。然而，活动期RA患者中SIRT2（1.73倍，p=0.065）和SIRT3（3.58倍，p=0.051）mRNA表达相对高于非活动期RA患者。
在RA中，SIRT3 mRNA表达增加，而SIRT2 mRNA表达降低。相反，活动期RA中SIRT2和SIRT3 mRNA表达增加。因此，在RA中每种SIRT的情况可能不同。
