Wang Yifeng, Hou Yuxuan, Qiu Jiehua, Li Zhiyong, Zhao Juan, Tong Xiaohong, Zhang Jian
State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China.
Int J Mol Sci. 2017 Jun 27;18(7):1376. doi: 10.3390/ijms18071376.
PKA (protein lysine acetylation) is a critical post-translational modification that regulates various developmental processes, including seed development. However, the acetylation events and dynamics on a proteomic scale in this process remain largely unknown, especially in rice early seed development. We report the first quantitative acetylproteomic study focused on rice early seed development by employing a mass spectral-based (MS-based), label-free approach. A total of 1817 acetylsites on 1688 acetylpeptides from 972 acetylproteins were identified in pistils and seeds at three and seven days after pollination, including 268 acetyproteins differentially acetylated among the three stages. Motif-X analysis revealed that six significantly enriched motifs, such as (DxkK), (kH) and (kY) around the acetylsites of the identified rice seed acetylproteins. Differentially acetylated proteins among the three stages, including adenosine diphosphate (ADP) -glucose pyrophosphorylases (AGPs), PDIL1-1 (protein disulfide isomerase like 1-1), hexokinases, pyruvate dehydrogenase complex (PDC) and numerous other regulators that are extensively involved in the starch and sucrose metabolism, glycolysis/gluconeogenesis, tricarboxylic acid (TCA) cycle and photosynthesis pathways during early seed development. This study greatly expanded the rice acetylome dataset, and shed novel insight into the regulatory roles of PKA in rice early seed development.
蛋白质赖氨酸乙酰化（PKA）是一种关键的翻译后修饰，可调节包括种子发育在内的各种发育过程。然而，在此过程中蛋白质组规模的乙酰化事件和动态变化仍 largely 未知，尤其是在水稻早期种子发育过程中。我们通过采用基于质谱（MS）的无标记方法，报道了第一项聚焦于水稻早期种子发育的定量乙酰蛋白质组学研究。在授粉后三天和七天的雌蕊和种子中，共鉴定出 972 种乙酰化蛋白质的 1688 条乙酰化肽段上的 1817 个乙酰化位点，其中包括在三个阶段中差异乙酰化的 268 种乙酰化蛋白质。Motif-X 分析显示，在已鉴定的水稻种子乙酰化蛋白质的乙酰化位点周围有六个显著富集的基序，如（DxkK）、（kH）和（kY）。三个阶段中差异乙酰化的蛋白质包括二磷酸腺苷（ADP）-葡萄糖焦磷酸化酶（AGP）、PDIL1-1（类蛋白质二硫键异构酶 1-1）、己糖激酶、丙酮酸脱氢酶复合体（PDC）以及许多其他在种子早期发育过程中广泛参与淀粉和蔗糖代谢、糖酵解/糖异生、三羧酸（TCA）循环和光合作用途径的调节因子。本研究极大地扩展了水稻乙酰化蛋白质组数据集，并为 PKA 在水稻早期种子发育中的调节作用提供了新的见解。
