非热等离子体对人结肠癌细胞的抗癌作用评估。
Evaluation of non-thermal plasma-induced anticancer effects on human colon cancer cells.
作者信息
Choi Jae-Sun, Kim Jeongho, Hong Young-Jun, Bae Woom-Yee, Choi Eun Ha, Jeong Joo-Won, Park Hun-Kuk
机构信息
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, South Korea.
Equal Contribution.
出版信息
Biomed Opt Express. 2017 Apr 26;8(5):2649-2659. doi: 10.1364/BOE.8.002649. eCollection 2017 May 1.
Abstract
Non-thermal atmospheric-pressure plasma has been introduced in various applications such as sterilization, wound healing, blood coagulation, and other biomedical applications. The most attractive application of non-thermal atmospheric-pressure plasma is in cancer treatment, where the plasma is used to produce reactive oxygen species (ROS) to facilitate cell apoptosis. We investigate the effects of different durations of exposure to dielectric-barrier discharge (DBD) plasma on colon cancer cells using measurement of cell viability and ROS levels, western blot, immunocytochemistry, and Raman spectroscopy. Our results suggest that different kinds of plasma-treated cells can be differentiated from control cells using the Raman data.
摘要
非热大气压等离子体已被应用于各种领域，如灭菌、伤口愈合、血液凝固及其他生物医学应用。非热大气压等离子体最具吸引力的应用是在癌症治疗中，其中等离子体用于产生活性氧（ROS）以促进细胞凋亡。我们使用细胞活力和ROS水平测量、蛋白质免疫印迹、免疫细胞化学和拉曼光谱，研究了不同暴露时长的介质阻挡放电（DBD）等离子体对结肠癌细胞的影响。我们的结果表明，使用拉曼数据可以将不同类型的经等离子体处理的细胞与对照细胞区分开来。
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