Rath Sibanarayan, Padhy Rabindra N
Central Research Laboratory, Institute of Medical Sciences (IMS) & Sum Hospital, Siksha 'O' Anusandhan University, Bhubaneswar, Odisha, India.
Integr Med Res. 2014 Sep;3(3):133-141. doi: 10.1016/j.imr.2014.04.002. Epub 2014 May 6.
To screen methanolic extracts of 26 commonly used Indian spices against nine species of uropathogenic bacteria (, , , , , , , , and ), isolated from clinical samples of a tertiary care hospital for antibacterial activity.
Bacterial strains were subjected to antibiotic sensitivity testing by Kirby-Bauer's disc diffusion method. Monitoring antibacterial potentiality of spice extracts was done by the agar-well diffusion method with multidrug resistant (MDR) strains of nine uropathogens.
The Gram-positive (GP) bacteria and were resistant to 16 of the 21 antibiotics used. Among the Gram-negative (GN) bacteria, resistant patterns were and to 12, to 14, to 12, to 10, to 11, and to 15 antibiotics of the 18 antibiotics used. The most effective 15 spices, having at least 25-29 mm as the size of the zone of inhibition, were , , , , , , , , , , , , , , and for at least one of the GP or GN MDR bacterial strains used. Moderate control capacity was registered by nine spices, , , , , , , , , and . However, the best two spices for controlling all the pathogens used were and , with the highest value of 29 mm as the inhibition zone size.
The most effective and unique 16 spice plants recorded for the control of MDR uropathogens could further be pursued for the development of complementary and supplementary medicine against MDR bacteria.
从一家三级护理医院的临床样本中分离出9种尿路致病性细菌(、、、、、、、和),筛选26种常用印度香料的甲醇提取物对这些细菌的抗菌活性。
采用 Kirby-Bauer 纸片扩散法对细菌菌株进行抗生素敏感性测试。通过琼脂孔扩散法,利用9种尿路病原体的多重耐药(MDR)菌株监测香料提取物的抗菌潜力。
革兰氏阳性(GP)细菌和对所使用的21种抗生素中的16种耐药。在革兰氏阴性(GN)细菌中,对所使用的18种抗生素的耐药模式分别为:对12种耐药,对14种耐药,对12种耐药,对10种耐药,对11种耐药,对15种耐药。对于至少一种所使用的 GP 或 GN MDR 细菌菌株,最有效的15种香料,其抑菌圈大小至少为25 - 29毫米,分别是、、、、、、、、、、、、、、和。9种香料,即、、、、、、、、和,显示出中等的控制能力。然而,对所有所使用病原体控制效果最佳的两种香料是和,其抑菌圈大小最高值为29毫米。
记录在案的对 MDR 尿路病原体控制最有效且独特的16种香料植物,可进一步用于开发针对 MDR 细菌的补充和替代药物。