Mathai Benan John, Meijer Annemarie H, Simonsen Anne
Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Sognsvannsveien 9, 0317 Oslo, Norway.
Institute of Biology Leiden, Leiden University, Einsteinweg 55, 2333 CC Leiden, The Netherlands.
Cells. 2017 Jul 9;6(3):21. doi: 10.3390/cells6030021.
Autophagy is an evolutionarily conserved catabolic process which allows lysosomal degradation of complex cytoplasmic components into basic biomolecules that are recycled for further cellular use. Autophagy is critical for cellular homeostasis and for degradation of misfolded proteins and damaged organelles as well as intracellular pathogens. The role of autophagy in protection against age-related diseases and a plethora of other diseases is now coming to light; assisted by several divergent eukaryotic model systems ranging from yeast to mice. We here give an overview of different methods used to analyse autophagy in zebrafish-a relatively new model for studying autophagy-and briefly discuss what has been done so far and possible future directions.
自噬是一种进化上保守的分解代谢过程，它允许溶酶体将复杂的细胞质成分降解为基本生物分子，这些生物分子可被循环利用以供细胞进一步使用。自噬对于细胞内稳态、错误折叠蛋白质和受损细胞器以及细胞内病原体的降解至关重要。在从酵母到小鼠等多种不同的真核模型系统的辅助下，自噬在预防与年龄相关的疾病和众多其他疾病中的作用正逐渐显现出来。我们在此概述了用于分析斑马鱼（一种研究自噬的相对较新的模型）自噬的不同方法，并简要讨论了到目前为止已经完成的工作以及可能的未来方向。
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