Skandalis Nicholas, Dimopoulou Anastasia, Georgopoulou Anthie, Gallios Nikolaos, Papadopoulos Dimitrios, Tsipas Dimitrios, Theologidis Ioannis, Michailidis Nikolaos, Chatzinikolaidou Maria
Department of Phytopathology, Benaki Phytopathological Institute, 8 St. Delta, Kifissia, GR-14561 Athens, Greece.
Department of Materials Science and Technology, University of Crete, 71001 Heraklion, Greece.
Nanomaterials (Basel). 2017 Jul 10;7(7):178. doi: 10.3390/nano7070178.
Silver nanoparticles (AgNPs) have been demonstrated to restrain bacterial growth, while maintaining minimal risk in development of bacterial resistance and human cell toxicity that conventional silver compounds exhibit. Several physical and chemical methods have been reported to synthesize AgNPs. However, these methods are expensive and involve heavy chemical reduction agents. An alternative approach to produce AgNPs in a cost-effective and environmentally friendly way employs a biological pathway using various plant extracts to reduce metal ions. The size control issue, and the stability of nanoparticles, remain some of the latest challenges in such methods. In this study, we used two different concentrations of fresh leaf extract of the plant (LEA) as a reducing and stabilizing agent to produce two size variations of AgNPs. UV-Vis spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy, and zeta potential were applied for the characterization of AgNPs. Both AgNP variations were evaluated for their antibacterial efficacy against the gram-negative species and , as well as the gram-positive species and . Although significant differences have been achieved in the nanoparticles' size by varying the plant extract concentration during synthesis, the antibacterial effect was almost the same.
银纳米颗粒（AgNPs）已被证明能够抑制细菌生长，同时在产生细菌耐药性和传统银化合物所表现出的人体细胞毒性方面保持最低风险。据报道，有几种物理和化学方法可用于合成AgNPs。然而，这些方法成本高昂且涉及重金属还原剂。一种以经济高效且环境友好的方式生产AgNPs的替代方法是采用生物途径，利用各种植物提取物来还原金属离子。纳米颗粒的尺寸控制问题以及稳定性仍然是此类方法面临的一些最新挑战。在本研究中，我们使用了两种不同浓度的植物新鲜叶提取物（LEA）作为还原剂和稳定剂，以制备两种不同尺寸的AgNPs。采用紫外可见光谱、动态光散射、透射电子显微镜和zeta电位对AgNPs进行表征。评估了两种AgNP变体对革兰氏阴性菌 和 以及革兰氏阳性菌 和 的抗菌效果。尽管在合成过程中通过改变植物提取物浓度实现了纳米颗粒尺寸的显著差异，但抗菌效果几乎相同。
