Zhang Ying, Guan Jing, Wu Jimin, Ding Sheng, Yang Jian, Zhang Jianhua, Dong Anjie, Deng Liandong
Department of Polymer Science and Technology, Key Laboratory of Systems Bioengineering of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China; Institute of Medical Support Technology, Academy of Military Sciences, Tianjin, 300161, China.
Research Center of Military Standard, Academy of Military Sciences, Beijing, 100141, China; Institute of Medical Support Technology, Academy of Military Sciences, Tianjin, 300161, China.
Carbohydr Polym. 2019 Sep 1;219:405-413. doi: 10.1016/j.carbpol.2019.05.028. Epub 2019 May 9.
N-alkylated chitosan (AC) sponges and graphene oxide (GO) sponges are promising candidates for emergency hemostat. However, AC sponges have weak mechanical strength and GO sponges may induce toxicity. To overcome these problems, a series of AC/GO composite spongs (ACGS) were prepared with various ratios (GO/AC, 0%, 5%, 10%, and 20%) using a dilute solution freeze phase separation and drying process. The sponges exhibit excellent absorption capacity, mechanical stability, and biocompatibility. In serial in vitro clotting tests, the higher the ratio of GO, the better the coagulation efficiency. ACGS with 20% ratio of GO (ACGS20) has shorter hemostatic time than Celox in a rabbit femoral injury test. Moreover, ACGS20 can accelerate erythrocyte and platelet adhesion. CD62p and intracellular Ca measurements show that ACGS20 can promote the release of intracellular Ca and stimulate platelet activation. These results suggest that ACGS20 is a good candidate composition for a safe and efficacious hemostatic dressing.
N-烷基化壳聚糖（AC）海绵和氧化石墨烯（GO）海绵是很有前景的紧急止血剂候选材料。然而，AC海绵机械强度较弱，而GO海绵可能会引发毒性。为克服这些问题，采用稀溶液冷冻相分离和干燥工艺制备了一系列不同比例（GO/AC，0%、5%、10%和20%）的AC/GO复合海绵（ACGS）。这些海绵表现出优异的吸收能力、机械稳定性和生物相容性。在一系列体外凝血试验中，GO比例越高，凝血效率越好。在兔股动脉损伤试验中，GO比例为20%的ACGS（ACGS20）止血时间比Celox短。此外，ACGS20能加速红细胞和血小板黏附。CD62p和细胞内钙测量结果表明，ACGS20能促进细胞内钙的释放并刺激血小板活化。这些结果表明，ACGS20是一种安全有效的止血敷料的良好候选成分。
