来自大肠杆菌K-12的γ-谷氨酰转肽酶：形成与定位
gamma-Glutamyltranspeptidase from Escherichia coli K-12: formation and localization.
作者信息
Suzuki H, Kumagai H, Tochikura T
出版信息
J Bacteriol. 1986 Dec;168(3):1332-5. doi: 10.1128/jb.168.3.1332-1335.1986.
Abstract
Escherichia coli cells showed maximum activity of gamma-glutamyltranspeptidase (EC 2.3.2.2) when they were grown at 20 degrees C, 14% of maximum activity at 37 degrees C, and none at 43 degrees C. The enzyme activity of intact cells grown at 20 degrees C was stably maintained after the temperature was changed to 45 degrees C. The activity increased during the exponential phase, and maximum activity was found at stationary phase. Its intracellular localization in the periplasmic space was confirmed.
摘要
大肠杆菌细胞在20℃培养时γ-谷氨酰转肽酶（EC 2.3.2.2）活性最高，在37℃时为最高活性的14%，在43℃时无活性。在20℃培养的完整细胞，当温度变为45℃后，酶活性仍能稳定维持。该酶活性在指数生长期增加，在稳定期达到最高。其在周质空间的细胞内定位得到了证实。
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