Venkatesan Jayachandran, Lowe Baboucarr, Manivasagan Panchanathan, Kang Kyong-Hwa, Chalisserry Elna P, Anil Sukumaran, Kim Dong Gyu, Kim Se-Kwon
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea.
Department of Marine-bio Convergence Science, Pukyong National University, Busan 608-737, Korea.
Materials (Basel). 2015 Aug 21;8(8):5426-5439. doi: 10.3390/ma8085253.
Nano-Hydroxyapatite (nHA) was isolated from salmon bone by alkaline hydrolysis. The resulting nHA was characterized using several analytical tools, including thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous. Microscopy demonstrated that the isolated nHA possessed a nanostructure with a size range of 6-37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) and was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. These results suggest that nHA derived from salmon is a promising biomaterial in the field of bone tissue engineering.
通过碱性水解从鲑鱼骨中分离出纳米羟基磷灰石（nHA）。使用多种分析工具对所得的nHA进行表征，包括热重分析（TGA）、傅里叶变换红外光谱（FT-IR）、X射线衍射分析（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM），以确定nHA样品的纯度。通过TGA证实了生鱼中有机物的去除。FT-IR证实了碳酸化基团的存在以及与合成Sigma HA的相似性。XRD显示分离出的nHA是无定形的。显微镜检查表明，分离出的nHA具有尺寸范围为6-37nm的纳米结构。所获得的nHA与间充质干细胞（MSCs）相互作用且无毒。与对照组相比，观察到nHA处理的MSCs矿化增加。这些结果表明，源自鲑鱼的nHA是骨组织工程领域中一种有前景的生物材料。
