College of Maritime Studies and Management, Chiang Mai University, Samut Sakhon 74000, Thailand.
College of Biomedical Engineering, Rangsit University, Pathumthani 12000, Thailand.
Int J Mol Sci. 2023 Feb 1;24(3):2776. doi: 10.3390/ijms24032776.
The optimum condition of acid hydrolysis for hydroxyapatite extraction from bigeye snapper () bone and the effects of extraction time (10-60 min) and HCl concentration (2.0-5.0% /) on yield and hydroxyapatite properties were determined. The optimum extracted condition was found using 5% HCl for 60 min, which was 13.4% yield; 19.8 g/100 g Ca content; 9.6 g/100 g P content; 2.1 Ca/P ratio; *, *, *; and Δ as 84.5, 2.8, 16.5, and 15.6, respectively. The using of 5% NaOH solution was optimum for hydroxyapatite precipitation from the extracted solution. The characteristic and biological properties of the obtained hydroxyapatite were studied. Fourier transform infrared spectroscopy and X-ray diffraction results showed a good comparison between the extracted and commercial hydroxyapatite. The microstructure of the extracted hydroxyapatite from a scanning electron microscope showed an irregular and flat-plate shape, large surface area, and roughness. The extracted hydroxyapatite was non- and low-cytotoxicity at a concentration of 50 and 100-400 µg/mL, respectively. Bovine serum albumin (BSA) adsorption and desorption of hydroxyapatite was studied. An increasing BSA concentration, hydroxyapatite amount, and adsorption time significantly increased protein adsorption on hydroxyapatite. Protein desorption from BSA-loaded hydroxyapatite showed an increase of release initially in the first 4 days and became a steady release rate until 14 days.
从大眼金枪鱼（）鱼骨中提取羟磷灰石的酸水解最佳条件以及提取时间（10-60 分钟）和 HCl 浓度（2.0-5.0% /）对产率和羟磷灰石性质的影响。通过使用 5% HCl 提取 60 分钟，确定了最佳提取条件，产率为 13.4%；钙含量为 19.8 g/100 g；磷含量为 9.6 g/100 g；Ca/P 比为 2.1；，，*；和Δ分别为 84.5、2.8、16.5 和 15.6。从提取溶液中沉淀羟磷灰石的最佳条件是使用 5% NaOH 溶液。研究了所得羟磷灰石的特性和生物性能。傅里叶变换红外光谱和 X 射线衍射结果表明，提取的和商业羟磷灰石之间具有良好的可比性。扫描电子显微镜显示提取的羟磷灰石的微观结构为不规则和平板形状，具有较大的表面积和粗糙度。在浓度为 50 和 100-400 µg/mL 时，提取的羟磷灰石是非毒性和低毒性的。研究了牛血清白蛋白（BSA）在羟磷灰石上的吸附和解吸。BSA 浓度、羟磷灰石量和吸附时间的增加显著增加了蛋白质在羟磷灰石上的吸附。从负载 BSA 的羟磷灰石中解吸蛋白质最初在最初的 4 天内显示出释放量的增加，然后变为稳定的释放速率，直到 14 天。
