Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran.
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran.
Int J Biol Macromol. 2018 Apr 15;110:416-424. doi: 10.1016/j.ijbiomac.2017.08.048. Epub 2017 Aug 9.
In this work, the synthesized graphene oxide/TiO/doxorubicin (GO/TiO/DOX) composites were loaded into the chitosan/poly(lactic acid) (PLA) solutions to fabricate the electrospun chitosan/PLA/GO/TiO/DOX nanofibrous scaffolds via electrospinning process. The synthesized composites and nanofibers were characterized using X-ray powder diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. Three-factor three-level central composite design was used to determine the influence of PLA to chitosan ratio, TiO/DOX content and GO/TiO/DOX content on the release of DOX from nanofibrous scaffolds. Drug loading efficiency and drug release behavior from nanofibers were investigated. The higher sustained release rate of DOX following the small burst release were achieved from nanofibrous scaffolds with 30 and 50μm thicknesses within two weeks incubation time. The faster DOX release rate from nanofibers was obtained in pH 5.3 compared to pH 7.4. Korsmayer-Peppas kinetic model was used to determine the DOX release mechanism from nanofibers. The cell viability results showed the higher proliferation inhibition effect of nanofibers on target lung cancer cells in the presence of magnetic field.
在这项工作中，将合成的氧化石墨烯/二氧化钛/阿霉素（GO/TiO/DOX）复合材料负载到壳聚糖/聚乳酸（PLA）溶液中，通过静电纺丝工艺制备壳聚糖/PLA/GO/TiO/DOX 纳米纤维支架。使用 X 射线粉末衍射（XRD）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析对合成的复合材料和纳米纤维进行了表征。采用三因素三水平中心复合设计来确定 PLA 与壳聚糖的比例、TiO/DOX 含量和 GO/TiO/DOX 含量对纳米纤维支架中 DOX 释放的影响。研究了纳米纤维的载药效率和药物释放行为。在两周的孵育时间内，厚度为 30 和 50μm 的纳米纤维支架具有较高的 DOX 突释后持续释放率，实现了较高的 DOX 释放率。与 pH7.4 相比，在 pH5.3 时，纳米纤维中的 DOX 释放速度更快。Korsmayer-Peppas 动力学模型用于确定纳米纤维中 DOX 的释放机制。细胞活力结果表明，在磁场存在的情况下，纳米纤维对靶肺癌细胞的增殖抑制作用更高。
