Squillaci Giuseppe, Parrella Roberta, Carbone Virginia, Minasi Paola, La Cara Francesco, Morana Alessandra
Institute of Agro-environmental and Forest Biology, National Research Council of Italy (CNR), Via Pietro Castellino 111, 80131, Naples, Italy.
Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Sciences, National Research Council of Italy (CNR), Via Roma 64, 83100, Avellino, Italy.
Extremophiles. 2017 Sep;21(5):933-945. doi: 10.1007/s00792-017-0954-y. Epub 2017 Aug 12.
Haloterrigena turkmenica was able to synthesize carotenoids when grown in halobacteria medium. These molecules have antioxidant properties and find application in food, cosmetic, and pharmaceutical fields. The carotenoids were extracted with methanol, separated by RP-HPLC, and identified by mass spectrometry and UV/Vis spectra analyses. The C carotenoids were the main pigments, and C, C, and C carotenoids were also detected. Seven geometric isomers were distinguished for bacterioruberin, monoanhydrobacterioruberin, and bisanhydrobacterioruberin. The assignment to a specific isomer was tentatively attempted through the analysis of the corresponding UV/Vis spectrum, the intensity of the cis peak, and its spectral fine structure. Lycopene, phytoene, and lycopersene were among the minor carotenoids further identified. The extract displayed antioxidant power higher than alpha-tocopherol, butylhydroxytoluene, and ascorbic acid used as reference compounds. Our studies identified for the first time seven geometric isomers of bacterioruberin derivatives and 30 carotenoids in a haloarchaeon.
土库曼嗜盐菌（Haloterrigena turkmenica）在嗜盐菌培养基中生长时能够合成类胡萝卜素。这些分子具有抗氧化特性，可应用于食品、化妆品和制药领域。类胡萝卜素用甲醇提取，通过反相高效液相色谱（RP-HPLC）分离，并通过质谱和紫外/可见光谱分析进行鉴定。C类胡萝卜素是主要色素，同时也检测到了C、C和C类胡萝卜素。对细菌红素、单脱水细菌红素和双脱水细菌红素区分出了七种几何异构体。通过分析相应的紫外/可见光谱、顺式峰的强度及其光谱精细结构，尝试初步确定特定的异构体。番茄红素、八氢番茄红素和番茄半萜也属于进一步鉴定出的次要类胡萝卜素。提取物显示出高于用作参考化合物的α-生育酚、丁基羟基甲苯和抗坏血酸的抗氧化能力。我们的研究首次在嗜盐古菌中鉴定出细菌红素衍生物的七种几何异构体和30种类胡萝卜素。
