Maan Preeti, Yadav Kuldeep Singh, Yadav Narayan Prasad
Department of Botany and Pharmacognosy, CSIR- Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, Uttar Pradesh, India.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S316-S320. doi: 10.4103/0973-1296.210163. Epub 2017 Jul 11.
The paste of stem bark of (AI) has been traditionally used on wound and scar for rapid healing in Bundelkhand region of India.
In the present investigation, wound healing potential of different extracts of stem bark of AI was explored in mice model.
To study the wound healing properties in small animal model, the excision and incision wound models were used and water, ethanol-water (1:1, v/v) and ethanol extracts were applied topically (15% w/w in ointment base). In the excision wound model, wound contraction, hydroxyproline content, DNA content, protein content, and nitric oxide levels were estimated after 14 days of topical treatment along with histopathological examinations. In the incision wound model, wound breaking strength was determined after 10 days of topical application of different extracts of AI.
The animals treated with water extract of AI exhibited significant increment in rate of wound contraction (93.39%, < 0.01), hydroxyproline content (13.31 ± 6.65 mg/g of dry tissue, < 0.001), DNA content (20.99 ± 0.68 μg/100 mg of tissue, < 0.01), protein content (100.53 ± 7.88 mg/g of wet tissue, < 0.01) and nitric oxide level (3.05 ± 0.03 mMol/g of tissue, < 0.001) as well as in wound breaking strength (289.40 ± 29.45 g, < 0.01) when compared with vehicle control group which was also supported by histopathological studies.
The water extract of stem bark of AI possesses significant wound healing property, validating its traditional use.
印度本德尔汗德地区传统上一直使用（AI）茎皮的糊剂来促进伤口和疤痕的快速愈合。
在本研究中，在小鼠模型中探究了AI茎皮不同提取物的伤口愈合潜力。
为了研究小动物模型中的伤口愈合特性，使用了切除和切开伤口模型，并局部应用水、乙醇 - 水（1:1，v/v）和乙醇提取物（在软膏基质中为15% w/w）。在切除伤口模型中，局部治疗14天后评估伤口收缩、羟脯氨酸含量、DNA含量、蛋白质含量和一氧化氮水平，并进行组织病理学检查。在切开伤口模型中，局部应用AI不同提取物10天后测定伤口抗张强度。
与赋形剂对照组相比，用AI水提取物处理的动物在伤口收缩率（93.39%，P < 0.01）、羟脯氨酸含量（13.31 ± 6.65 mg/g干组织，P < 0.001）、DNA含量（20.99 ± 0.68 μg/100 mg组织，P < 0.01）、蛋白质含量（100.53 ± 7.88 mg/g湿组织，P < 0.01）和一氧化氮水平（3.05 ± 0.03 mMol/g组织，P < 0.001）以及伤口抗张强度（289.40 ± 29.45 g，P < 0.01）方面均表现出显著增加，组织病理学研究也支持这一结果。
AI茎皮的水提取物具有显著的伤口愈合特性，证实了其传统用途。
