Sun Chuanhui, Han Changsong, Wang Peng, Jin Yinji, Sun Yanan, Qu Lingmei
Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.
Department of Pathology, Harbin Medical University, Harbin 150081, China.
Biomed Res Int. 2017;2017:3680305. doi: 10.1155/2017/3680305. Epub 2017 Jul 20.
The purpose of this study was to investigate the HOX gene expression profile in laryngeal squamous cell carcinoma (LSCC) and assess whether some genes are associated with the clinicopathological features and prognosis in LSCC patients. The HOX gene levels were tested by microarray and validated by qRT-PCR in paired cancerous and adjacent noncancerous LSCC tissue samples. The microarray testing data of 39 HOX genes revealed 15 HOX genes that were at least 2-fold upregulated and 2 that were downregulated. After qRT-PCR evaluation, the three most upregulated genes (HOXB9, HOXB13, and HOXD13) were selected for tissue microarray (TMA) analysis. The correlations between the HOXB9, HOXB13, and HOXD13 expression levels and both clinicopathological features and prognosis were analyzed. Three HOX gene expression levels were markedly increased in LSCC tissues compared with adjacent noncancerous tissues ( < 0.001). HOXB9 was found to correlate with histological grade ( < 0.01) and prognosis ( < 0.01) in LSCC. In conclusion, this study revealed that HOXB9, HOXB13, and HOXD13 were upregulated and may play important roles in LSCC. Moreover, HOXB9 may serve as a novel marker of poor prognosis and a potential therapeutic target in LSCC patients.
本研究的目的是调查喉鳞状细胞癌（LSCC）中HOX基因的表达谱，并评估某些基因是否与LSCC患者的临床病理特征及预后相关。通过微阵列检测HOX基因水平，并在配对的LSCC癌组织和癌旁非癌组织样本中用qRT-PCR进行验证。39个HOX基因的微阵列检测数据显示，有15个HOX基因上调至少2倍，2个HOX基因下调。经qRT-PCR评估后，选择上调最明显的三个基因（HOXB9、HOXB13和HOXD13）进行组织芯片（TMA）分析。分析HOXB9、HOXB13和HOXD13表达水平与临床病理特征及预后之间的相关性。与癌旁非癌组织相比，LSCC组织中三个HOX基因的表达水平显著升高（<0.001）。发现HOXB9与LSCC的组织学分级（<0.01）及预后（<0.01）相关。总之，本研究表明HOXB9、HOXB13和HOXD13上调，可能在LSCC中起重要作用。此外，HOXB9可能作为LSCC患者预后不良的新标志物和潜在治疗靶点。
