Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), San 31, Hyoja-dong, Pohang, 790-784, Republic of Korea.
Ann Biomed Eng. 2017 Nov;45(11):2563-2573. doi: 10.1007/s10439-017-1902-9. Epub 2017 Aug 18.
Transfusion is crucial in surgical operation and anemia treatment. However, several hemorheological properties of blood are adversely altered during blood storage. After transfusion, these adverse alterations are related with decrease of oxygen and ion transport in blood circulation, which increase the mortality of transfused patients. Therefore, accurate sensing of whether a blood supply is still viable for transfusion or not is extremely important. In this study, a H-shaped microfluidic device and digital in-line holographic microscopy were employed to measure temporal variations of blood viscosity and the optical focusing property of erythrocytes during blood storage. Stored rat blood samples separately preserved in citrate phosphate dextrose adenine-1 (CPDA-1) and ethylenediaminetetraacetic acid (EDTA) underwent considerable changes in their biophysical parameters after 2 weeks. Compared with EDTA, CPDA-1 preserves the hemorheological properties of stored blood more effectively. We propose new criteria for depository period of stored blood and indexes, such as viscosity and focal length of erythrocytes, to determine its appropriateness for transfusion. These criteria and indexes can be effectively used for high-throughput prescreening to reduce the risk of transfusion of aged blood or diagnose hematological diseases.
输血在手术和贫血治疗中至关重要。然而，血液的一些血液流变学特性在血液储存过程中会发生不利变化。输血后，这些不利变化与血液循环中氧气和离子运输的减少有关，从而增加了输血患者的死亡率。因此，准确感知血液供应是否仍然适合输血非常重要。在这项研究中，使用 H 形微流控装置和数字线阵全息显微镜来测量血液储存过程中血液粘度的时间变化和红细胞的光学聚焦特性。分别保存在柠檬酸盐磷酸盐葡萄糖腺嘌呤-1（CPDA-1）和乙二胺四乙酸（EDTA）中的储存大鼠血液样本在 2 周后其生物物理参数发生了相当大的变化。与 EDTA 相比，CPDA-1 更有效地保存了储存血液的血液流变学特性。我们提出了储存血液的储存期的新标准和指标，例如红细胞的粘度和焦距，以确定其输血的适宜性。这些标准和指标可有效用于高通量预筛选，以降低输血老化血液或诊断血液疾病的风险。
