Ma Xiao-Jun, Mo Chang-Ming
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medical Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(11):2021-2031. doi: 10.19540/j.cnki.cjcmm.2017.0087.
The molecular-assisted breeding, transgenic breeding and molecular designing breeding are three development directions of plant molecular breeding. Base on these three development directions, this paper summarizes developing status and new tendency of research field of genetic linkage mapping, QTL mapping, association mapping, molecular-assisted selections, pollen-mediated transformations, agrobacterium-mediated transformations, particle gun-mediated transformations, genome editing technologies, whole-genome sequencing, transcriptome sequencing, proteome sequencing and varietal molecular designing. The objective and existing problem of medical plant molecular breeding were discussed the prospect of these three molecular breeding technologies application on medical plant molecular breeding was outlooked.
分子辅助育种、转基因育种和分子设计育种是植物分子育种的三个发展方向。基于这三个发展方向，本文综述了遗传连锁图谱构建、QTL定位、关联分析、分子辅助选择、花粉介导转化、农杆菌介导转化、基因枪介导转化、基因组编辑技术、全基因组测序、转录组测序、蛋白质组测序以及品种分子设计等研究领域的发展现状和新趋势。讨论了药用植物分子育种的目标和存在的问题，并展望了这三种分子育种技术在药用植物分子育种中的应用前景。
