Department of Biochemistry, Parc Taulí Hospital Universitari. Institut d'Investigació I Innovació Parc Taulí I3PT, Universitat Autònoma de Barcelona, Sabadell, Spain.
Cytometry B Clin Cytom. 2018 Sep;94(5):680-688. doi: 10.1002/cyto.b.21587. Epub 2017 Aug 31.
Hematological cytometers with a biological fluid module could potentially correct the limitations of the manual chamber method. This study evaluates the agreement between the manual technique and the Sysmex XN-1000 analyzer for white blood cell (WBC) and red blood cell (RBC) counts, as well as for leukocyte differentiation in different types of fluids. This study also evaluates the advantages of incorporating the technique in routine laboratory work.
One hundred and three fluid samples examined were 45 ascite (AF), 21 synovial (SF), 33 pleural (PF), and 31 cerebrospinal (CSF) fluid samples. All cell counting was performed with a Sysmex XN-1000 and a Fuchs-Rosenthal counting chamber. May Gründwald-Giemsa stain was used for manual WBC differentiation. The manual analysis data were obtained in duplicate by the same two observers. Passing-Bablok regression and the Kappa index were used to evaluate the interchangeability and concordance.
Good agreement was observed for WBC differentiation in all fluids and for WBC counts in SF and PF. An optimal Kappa index was obtained, which indicated agreement and clinical significance for WBC and RBC counts in CSF and for RBC counts in PF. There was disagreement for WBC and RBC analysis in AF, with significantly higher results from the Sysmex XN-1000 than from the manual method. A reduction in laboratory response time was observed when using the automatic method.
Except for AF, the Sysmex XN-1000 results agree with those of the manual method, although to different degrees depending on the fluid type. © 2017 International Clinical Cytometry Society.
具有生物流体模块的血液学细胞计数器有可能纠正手动室方法的局限性。本研究评估了手动技术与 Sysmex XN-1000 分析仪在不同类型液体中的白细胞 (WBC) 和红细胞 (RBC) 计数以及白细胞分化方面的一致性。本研究还评估了将该技术纳入常规实验室工作的优势。
检查了 103 个液体样本，其中包括 45 个腹水 (AF)、21 个滑液 (SF)、33 个胸腔积液 (PF) 和 31 个脑脊液 (CSF) 样本。所有细胞计数均使用 Sysmex XN-1000 和 Fuchs-Rosenthal 计数室进行。手工 WBC 分化采用 May Gründwald-Giemsa 染色。手动分析数据由同两名观察者重复获得。通过 Passing-Bablok 回归和 Kappa 指数评估互换性和一致性。
在所有液体中均观察到 WBC 分化良好，在 SF 和 PF 中观察到 WBC 计数良好。获得了最佳的 Kappa 指数，这表明 CSF 中的 WBC 和 RBC 计数以及 PF 中的 RBC 计数具有一致性和临床意义。在 AF 中，WBC 和 RBC 分析存在分歧，XN-1000 的结果明显高于手动方法。使用自动方法时，实验室反应时间缩短。
除了 AF 之外，XN-1000 的结果与手动方法一致，尽管根据液体类型的不同，一致性程度有所不同。
