Fernandez Ana Claudia A M, Rosa Maurício F, Fernandez Carla M M, C Bortolucci Wanessa, Melo Ulisses Z, Siqueira Vera L D, Cortez Diógenes A G, Gonçalves José E, Linde Giani A, Gazim Zilda C
Pharmaceutical Sciences, State University of Western Paraná, Cascavel, Paraná, 85.819-110, Brazil.
Pharmaceutical Sciences, State University of Maringá, Maringá, Paraná, 87020-900, Brazil.
Curr Microbiol. 2017 Dec;74(12):1453-1460. doi: 10.1007/s00284-017-1340-9. Epub 2017 Aug 24.
Tetradenia riparia (Lamiaceae) is native to Central Africa popularly known as myrrh, used in folk medicine to treat various diseases like malaria, gastroenteritis, and tropical skin disease. This research was to evaluate the antioxidant and antibacterial activities of the crude extract (CE) and fractions (FR) of the T. riparia by classical chromatography. The CE of T. riparia leaves was submitted to column chromatographic fractionation to obtain four fractions of the interest, which were identified by nuclear magnetic resonance and gas chromatograph coupled to mass spectrum: FR-I (abieta-7,9(11)-dien-13-β-ol), FR-II (Ibozol), FR-III (8 (14), 15-sandaracopimaradiene-2α, 18-diol and 8 (14), 15-sandaracopimaradiene-7α, 18-diol), and FR-IV (Astragalin, Boronolide and Luteolin). Total phenol content of CE and FR were measured, and antioxidant action by methods of DPPH (2,2-diphenyl-1-picrylhydrazyl), β-carotene/linoleic acid system, and ferric reducing/antioxidant power (FRAP) and the antibacterial activity was evaluated by the broth microdilution method with the determination of the minimum inhibitory concentration (MIC). The FR-IV presented antioxidant potential with 181.67 μg gallic acid/mg, IC of 0.61 μg/mL by DPPH method, 55.61% oxidation protection by β-carotene/linoleic acid system and 4.59 µM ferrous sulfate/mg of sample by FRAP, and the FR-I showed higher antibacterial potential on the strain Staphylococcus aureus with MIC 0.98 μg/mL, Enterococcus faecalis and Bacillus cereus with MIC 31.2 μg/mL. Thus, the fractionation of CE was extremely important to detect fractions with potential activities, and investigations are necessary regarding the mechanism of action and action in vivo.
垂枝香茶菜（唇形科）原产于中非，俗称没药，在民间医学中用于治疗多种疾病，如疟疾、肠胃炎和热带皮肤病。本研究旨在通过经典色谱法评估垂枝香茶菜粗提物（CE）和馏分（FR）的抗氧化和抗菌活性。垂枝香茶菜叶片的粗提物进行柱色谱分离，得到四个目标馏分，通过核磁共振和气相色谱 - 质谱联用进行鉴定：FR - I（枞-7,9(11)-二烯-13-β-醇）、FR - II（伊波醇）、FR - III（8(14),15-山达海松二烯-2α,18-二醇和8(14),15-山达海松二烯-7α,18-二醇）以及FR - IV（紫云英苷、硼诺内酯和木犀草素）。测定了粗提物和馏分的总酚含量，并通过DPPH（2,2-二苯基-1-苦基肼）法、β-胡萝卜素/亚油酸体系和铁还原/抗氧化能力（FRAP）法评估抗氧化作用，通过肉汤微量稀释法测定最低抑菌浓度（MIC）来评估抗菌活性。FR - IV表现出抗氧化潜力，其没食子酸含量为181.67μg/mg，DPPH法的IC50为0.61μg/mL，β-胡萝卜素/亚油酸体系的氧化保护率为55.61%，FRAP法为4.59μM硫酸亚铁/mg样品，而FR - I对金黄色葡萄球菌菌株的抗菌潜力更高，MIC为0.98μg/mL，对粪肠球菌和蜡样芽孢杆菌的MIC为31.2μg/mL。因此，粗提物的分离对于检测具有潜在活性的馏分极为重要，关于其作用机制和体内作用的研究很有必要。
