Department of BioEngineering, Lehigh University, Bethlehem, Pennsylvania 18015, USA.
Lab Chip. 2017 Sep 26;17(19):3291-3299. doi: 10.1039/c7lc00333a.
A wavy-herringbone (wavy-HB) structured microfluidic device was used to effectively and selectively capture and release circulating tumor cells (CTCs) by using immunoaffinity and magnetic force. This device was designed to create passive turbulence and increase the possibility of tumor cells colliding with the device wall. Under an external magnetic field, magnetic particles (MPs) coated with anti-EpCAM against a tumor cell surface protein (EpCAM) were immobilized over the wavy-HB surface to capture tumor cells. After removing the magnetic field, the captured cells with surplus MPs were released from the device and collected; thus, these cells could be re-cultured for further analysis. Under optimized conditions, the capture efficiency of the tumor cells can be as high as 92% ± 2.8%. Capture experiments were also performed on whole blood samples, and the capture efficiency was in a high range of 81-95%, at different tumor cell concentrations. Such a method can potentially be used for CTC sorting from patient blood samples, CTC concentration monitoring, therapeutic guidance and drug dosage choice, and further study of tumors, such as drug screening and tumor mutations.
一种波纹-人字形（wavy-HB）结构的微流控装置被用于通过免疫亲和性和磁力有效地和选择性地捕获和释放循环肿瘤细胞（CTCs）。该装置旨在产生被动湍流，并增加肿瘤细胞与装置壁碰撞的可能性。在外磁场的作用下，涂有针对肿瘤细胞表面蛋白（EpCAM）的抗-EpCAM 的磁性颗粒（MPs）固定在波纹-HB 表面以捕获肿瘤细胞。去除磁场后，从装置上释放并收集带有多余 MPs 的捕获细胞；因此，可以对这些细胞进行再培养以进行进一步分析。在优化条件下，肿瘤细胞的捕获效率高达 92%±2.8%。在不同的肿瘤细胞浓度下，对全血样本也进行了捕获实验，捕获效率在 81-95%的高范围内。这种方法有可能用于从患者的血液样本中分离 CTC、CTC 浓度监测、治疗指导和药物剂量选择，以及进一步研究肿瘤，如药物筛选和肿瘤突变。
