Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charitéplatz 1, 10117 Berlin, Germany; Kim Il Sung University, Ryongnam-Dong, Taesong District, Pyongyang, Democratic People's Republic of Korea.
Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Charitéplatz 1, 10117 Berlin, Germany.
Mech Ageing Dev. 2018 Jun;172:6-12. doi: 10.1016/j.mad.2017.08.011. Epub 2017 Aug 24.
In this study, stratum corneum (SC) depth profiles of hydrogen bound water molecule types, intercellular lipid (ICL) ordering, concentration of natural moisturizing factor (NMF) and keratin folding/unfolding properties are investigated in vivo for older (mean 50 years old) and younger (mean 29 years old) human skin using confocal Raman microscopy. The results show that the SC of the older group is modestly thicker (p<0.1), has more hydrogen bound water molecules at the depth 10-30% of the SC thickness (p<0.05), has a higher ordered organization of ICL (p<0.1) and higher concentration of NMF (p<0.05) at the depth 20-40% of the SC thickness compared to the younger group. This study also reveals, that the hydrogen bonding state of water highly correlates with NMF and the lateral structure of ICL but not with keratin's folding/unfolding properties. The presented results let suggest, that the decreased trans-epidermal water loss (TEWL) with increasing age cannot be sufficiently explained by only the increased SC thickness, but additionally by the increase of ICL ordering, higher NMF concentration and thus larger amount of hydrogen bound water molecules at the depth 20-40% of the SC thickness.
本研究采用共聚焦拉曼显微镜，对老年人（平均年龄 50 岁）和年轻人（平均年龄 29 岁）的皮肤，分别进行角质层（SC）中氢键水分子类型、细胞间脂质（ICL）有序性、天然保湿因子（NMF）浓度和角蛋白折叠/展开特性的研究。结果表明，与年轻组相比，老年组的 SC 略厚（p<0.1），在 SC 厚度的 10-30%深度处有更多的氢键水分子（p<0.05），在 SC 厚度的 20-40%深度处 ICL 的有序组织更高（p<0.1），NMF 浓度更高（p<0.05）。本研究还揭示，水的氢键状态与 NMF 和 ICL 的层状结构高度相关，但与角蛋白的折叠/展开特性无关。研究结果表明，随着年龄的增长，经表皮水分流失（TEWL）减少不能仅归因于 SC 厚度的增加，还归因于 ICL 有序性增加、NMF 浓度增加以及 SC 厚度的 20-40%深度处氢键水分子数量增加。
