Traavik T, Mehl R, Berdal B P, Lund S, Dalrymple J M
Scand J Infect Dis. 1983;15(1):11-6. doi: 10.3109/inf.1983.15.issue-1.03.
In search for the Norwegian nephropathia epidemica (NE) agent, lung sections from small rodents and sera from human patients have been tested by indirect fluorescent antibody technique (IFAT). A cytoplasmatic antigen reacting with patient sera and a Korean hemorrhagic fever (KHF) reference antiserum was found in the lungs of 6 bank voles (Clethrionomys glareolus) from 2 different locations. Antibodies reacting with vole lung antigen (NE agent) and KHF agent in A-549 cells were detected in sera from 35/57 patients clinically suspected of having NE. Specific IgM antibodies were usually detected in "early" obtained sera. Positive sera had considerably higher titers against NE than KHF agent. One serum had an IgG titer of 160 against NE, but no detectable KHF antibodies. One patient had an atypical disease. Two NE patients must have been infected north of the distribution area for Cl. glareolus, suggesting the existence of other reservoir animals.
为寻找挪威流行性肾病（NE）病原体，采用间接荧光抗体技术（IFAT）检测了小型啮齿动物的肺组织切片和人类患者的血清。在来自2个不同地点的6只棕背䶄（Clethrionomys glareolus）的肺中发现了一种与患者血清及韩国出血热（KHF）参考抗血清发生反应的细胞质抗原。在临床上疑似患有NE的57例患者中的35例血清中检测到了与田鼠肺抗原（NE病原体）及A-549细胞中的KHF病原体发生反应的抗体。特异性IgM抗体通常在“早期”采集的血清中检测到。阳性血清针对NE的滴度明显高于针对KHF病原体的滴度。一份血清针对NE的IgG滴度为160，但未检测到KHF抗体。一名患者患有非典型疾病。两名NE患者肯定是在棕背䶄分布区域以北感染的，这表明存在其他储存宿主动物。
