基于显微镜物镜的4光谱组织散射测角法。
Microscope objective based 4 spectroscopic tissue scattering goniometry.
作者信息
Simmons Z J, Rogers J D
机构信息
Department of Biomedical Engineering, University of Wisconsin-Madison, 1550 Engineering Drive, Madison, WI 53706, USA.
McPherson Eye Research Institute, University of Wisconsin-Madison, 1111 Highland Avenue, WIMR 9433, Madison, WI 53705, USA.
出版信息
Biomed Opt Express. 2017 Jul 25;8(8):3828-3841. doi: 10.1364/BOE.8.003828. eCollection 2017 Aug 1.
The measurement of optical scattering as a function of angle, goniometry, can provide a wealth of information about tissue. The goniometry technique described here measures the intensity profile at the pupil planes of two microscope objectives with a scattering sample between them. The maximum observable scattering angle is extended by employing off-axis illumination. This configuration permits several advantages including: i) rapid measurement of scattering into 4 sr to characterize the entire scattering phase function in isotropic tissue, ii) sensitivity to axially asymmetric scattering from anisotropic fibrous tissue, iii) selective interrogation of small regions within spatially inhomogenous tissue, iv) concurrent measurement of scattering coefficient , and v) measurement of wavelength dependent scattering properties via spectrally tunable source. The instrument is validated by comparing measurements of microsphere suspensions to the Mie scattering solution. Instrument capabilities are demonstrated with samples of rat brain and mouse eye tissues.
作为角度函数的光学散射测量，即测角术，可以提供关于组织的大量信息。这里描述的测角术技术测量两个显微镜物镜光瞳平面处的强度分布，中间放置一个散射样本。通过采用离轴照明来扩展最大可观测散射角。这种配置具有几个优点，包括：i）快速测量进入4球面度的散射，以表征各向同性组织中的整个散射相函数；ii）对各向异性纤维组织的轴向不对称散射敏感；iii）对空间不均匀组织内的小区域进行选择性询问；iv）同时测量散射系数；v）通过光谱可调光源测量波长相关的散射特性。通过将微球悬浮液的测量结果与米氏散射解进行比较，对该仪器进行了验证。用大鼠脑和小鼠眼组织样本展示了仪器的功能。
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