Department of Nanobiomedical Science, Dankook University, Cheonan, 31116, Republic of Korea.
Department of Pharmaceutical Engineering, Dankook University, Cheonan, 31116, Republic of Korea.
J Biomed Mater Res A. 2018 Jan;106(1):52-64. doi: 10.1002/jbm.a.36216. Epub 2017 Sep 23.
In this study, we fabricated a nerve guide conduit (NGC) with nerve growth factor (NGF) gradient along the longitudinal direction by rolling a porous polycaprolactone membrane with NGF concentration gradient. The NGF immobilized on the membrane was continuously released for up to 35 days, and the released amount of the NGF from the membrane gradually increased from the proximal to distal NGF ends, which may allow a neurotrophic factor gradient in the tubular NGC for a sufficient period. From the in vitro cell culture experiment, it was observed that the PC12 cells sense the NGF concentration gradient on the membrane for the cell proliferation and differentiation. From the in vivo animal experiment using a long gap (20 mm) sciatic nerve defect model of rats, the NGC with NGF concentration gradient allowed more rapid nerve regeneration through the NGC than the NGC itself and NGC immobilized with uniformly distributed NGF. The NGC with NGF concentration gradient seems to be a promising strategy for the peripheral nerve regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 52-64, 2018.
在这项研究中，我们通过将具有神经生长因子（NGF）浓度梯度的多孔聚己内酯膜滚压来制造具有沿纵向的 NGF 浓度梯度的神经引导管（NGC）。固定在膜上的 NGF 持续释放长达 35 天，并且从近端到远端 NGF 端，NGF 的释放量逐渐增加，这可能允许管状 NGC 中的神经营养因子梯度持续足够长的时间。从体外细胞培养实验中观察到，PC12 细胞能够感知到膜上的 NGF 浓度梯度，从而促进细胞增殖和分化。在使用大鼠 20mm 坐骨神经缺损模型的体内动物实验中，具有 NGF 浓度梯度的 NGC 允许通过 NGC 进行更快的神经再生，而不是 NGC 本身和固定有均匀分布的 NGF 的 NGC。具有 NGF 浓度梯度的 NGC 似乎是外周神经再生的一种很有前途的策略。©2017 Wiley Periodicals, Inc. J 生物材料 Res 部分 A：106A：52-64，2018。
