Srisatjaluk Ratchapin L, Klongnoi Boworn, Wongsirichat Natthamet
Department of Oral Microbiology Faculty of Dentistry Mahidol University, Bangkok, Thailand.
Department Oral & Maxillofacial Surgery, Faculty of Dentistry Mahidol University, Bangkok, Thailand.
J Dent Anesth Pain Med. 2016 Mar;16(1):17-24. doi: 10.17245/jdapm.2016.16.1.17. Epub 2016 Mar 31.
To evaluate the antimicrobial activity of lidocaine (LD) topical anesthetic spray against oral microflora.
Antimicrobial effects of 10% LD spray were assessed against six bacterial cultures obtained from volunteers: , , , , , and . The filter papers contained 50-µl LD, brain heart infusion (BHI) broth, or 0.2% chlorhexidine. Papers were placed on the cultured blood plates for 1-3 min. After the papers were removed, plates were incubated for 24 h. Bacterial growth on the contact areas was recorded as the antimicrobial score. The split mouth technique was use in for sample collection in clinical study. Filter papers soaked with either BHI broth or LD were placed on the right or left buccal mucosa for 1 min, and replaced with other papers to imprint biofilms onto the contact areas. Papers were placed on blood plates, incubated for 24 h, and antimicrobial scores were determined. Experiments were conducted for 2- and 3-min exposure times with a 1-day washout period.
LD exhibited bactericidal effects against , , and within 1 min but displayed no effect against , , and . The antimicrobial effect of LD on oral microflora depended upon exposure time, similar to the results obtained from the clinical study (P < 0.05). LD showed 60-95% biofilm reduction on buccal mucosa.
Antimicrobial activity of 10% LD topical anesthetic spray was increased by exposure time. The 3 min application reduced oral microflora in the buccal mucosa.
评估利多卡因（LD）局部麻醉喷雾剂对口腔微生物群的抗菌活性。
评估10% LD喷雾剂对从志愿者获得的六种细菌培养物的抗菌效果：[此处六种细菌名称未给出]。滤纸片含有50μl LD、脑心浸液（BHI）肉汤或0.2%氯己定。将滤纸片放置在培养的血平板上1 - 3分钟。取出滤纸片后，将平板培养24小时。记录接触区域的细菌生长情况作为抗菌评分。临床研究采用双侧对照技术进行样本采集。用BHI肉汤或LD浸泡的滤纸片放置在右侧或左侧颊黏膜上1分钟，然后更换为其他滤纸片以将生物膜印在接触区域。将滤纸片放置在血平板上，培养24小时，并确定抗菌评分。实验在暴露时间为2分钟和3分钟且洗脱期为1天的条件下进行。
LD在1分钟内对[此处三种细菌名称未给出]表现出杀菌作用，但对[此处三种细菌名称未给出]没有作用。LD对口腔微生物群的抗菌作用取决于暴露时间，这与临床研究结果相似（P < 0.05）。LD使颊黏膜上的生物膜减少60 - 95%。
10% LD局部麻醉喷雾剂的抗菌活性随暴露时间增加。3分钟的应用减少了颊黏膜中的口腔微生物群。
