Abdollahzadeh Sh, Mashouf Ry, Mortazavi H, Moghaddam Mh, Roozbahani N, Vahedi M
Assistant Professor, Department of Oral Medicine, School of Dentistry, Hamadan University of Medical Sciences, Hamadan, Iran.
J Dent (Tehran). 2011 Winter;8(1):1-6. Epub 2011 Mar 31.
Punica granatum has been used for many years in folk medicine due to several purposes. The aim of the present study was to evaluate the effect of methanolic extract of Punica granatum peel (MEPGP) against Streptococcus mutans, Staphylococcus aureus, Streptococcus salivarius, Streptococcus sanguinis, Staphylococcus epidermidis, Actynomyces viscosus, Lactobacillus acidophilus and Candida albicans.
In this in vitro study, the mentioned oral organisms were cultured in blood agar and mueller-hinton media and then paper disks containing MEPGP at concentrations of 4 mg/ml, 8 mg/ml and 12 mg/ml were inserted on medias. The antimicrobial activity was evaluated by agar disk diffusion method. The effects of three different concentrations of MEPGP against microorganisms were compared using one-way ANOVA and Tukey tests.
All concentrations of MEPGP had antibacterial activity against S. aureus and S. epidermidis. Only at concentration of 8 mg/ml and 12 mg/ml MEPGP was effective against L. acidophilus, S. mutans and S. salivarius. Furthermore; no concentrations of MEPGP inhibited A. viscosus and C. albicans.
This study suggests that MEPGP might be used as an antibacterial agent in controlling oral infections.
石榴因其多种用途在民间医学中已被使用多年。本研究的目的是评估石榴皮甲醇提取物（MEPGP）对变形链球菌、金黄色葡萄球菌、唾液链球菌、血链球菌、表皮葡萄球菌、粘性放线菌嗜酸乳杆菌和白色念珠菌的作用。
在这项体外研究中，将上述口腔微生物在血琼脂和穆勒-欣顿培养基中培养，然后将含有浓度为4mg/ml、8mg/ml和12mg/ml的MEPGP的纸片插入培养基中。通过琼脂纸片扩散法评估抗菌活性。使用单向方差分析和Tukey检验比较三种不同浓度的MEPGP对微生物的影响。
所有浓度的MEPGP对金黄色葡萄球菌和表皮葡萄球菌均有抗菌活性。仅在8mg/ml和12mg/ml浓度下，MEPGP对嗜酸乳杆菌、变形链球菌和唾液链球菌有效。此外，没有浓度的MEPGP能抑制粘性放线菌和白色念珠菌。
本研究表明，MEPGP可能作为一种抗菌剂用于控制口腔感染。
