Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 220-701, Republic of Korea.
Department of Microbiology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea.
Oxid Med Cell Longev. 2017;2017:7340143. doi: 10.1155/2017/7340143. Epub 2017 Aug 13.
As fullerene-based compound applications have been rapidly increasing in the health industry, the need of biomedical research is urgently in demand. While shungite is regarded as a natural source of fullerene, it remains poorly documented. Here, we explored the effects of shungite against ultraviolet B- (UVB-) induced skin damage by investigating the physiological skin parameters, immune-redox profiling, and oxidative stress molecular signaling. Toward this, mice were UVB-irradiated with 0.75 mW/cm for two consecutive days. Consecutively, shungite was topically applied on the dorsal side of the mice for 7 days. First, we found significant improvements in the skin parameters of the shungite-treated groups revealed by the reduction in roughness, pigmentation, and wrinkle measurement. Second, the immunokine profiling in mouse serum and skin lysates showed a reduction in the proinflammatory response in the shungite-treated groups. Accordingly, the redox profile of shungite-treated groups showed counterbalance of ROS/RNS and superoxide levels in serum and skin lysates. Last, we have confirmed the involvement of Nrf2- and MAPK-mediated oxidative stress pathways in the antioxidant mechanism of shungite. Collectively, the results clearly show that shungite has an antioxidant and anti-inflammatory action against UVB-induced skin damage in hairless mice.
随着富勒烯类化合物在健康产业中的应用迅速增加，对生物医学研究的需求也迫在眉睫。虽然石墨烯被认为是富勒烯的天然来源，但它的相关研究仍鲜有记载。在这里，我们通过研究生理皮肤参数、免疫氧化还原谱和氧化应激分子信号来探讨石墨烯对紫外线 B （UVB）诱导的皮肤损伤的影响。为此，我们用 0.75mW/cm 的 UVB 连续照射小鼠两天。然后，我们将石墨烯涂在小鼠背部连续 7 天。首先，我们发现，通过减少粗糙度、色素沉着和皱纹测量，石墨烯处理组的皮肤参数得到了显著改善。其次，小鼠血清和皮肤裂解物中的免疫细胞因子谱显示，石墨烯处理组的促炎反应减少。相应地，石墨烯处理组的氧化还原谱显示，血清和皮肤裂解物中的 ROS/RNS 和超氧化物水平得到了平衡。最后，我们证实了 Nrf2 和 MAPK 介导的氧化应激途径参与了石墨烯的抗氧化机制。总的来说，这些结果清楚地表明，石墨烯对无毛小鼠的 UVB 诱导的皮肤损伤具有抗氧化和抗炎作用。
