Adány R, Glukhova M A, Kabakov A Y, Muszbek L
Department of Clinical Chemistry, University School of Medicine, Debrecen, Hungary.
J Clin Pathol. 1988 Jan;41(1):49-56. doi: 10.1136/jcp.41.1.49.
Paraffin embedded sections of human liver, lymph node, and placenta showed that certain connective tissue cells were positive for factor XIII subunit a. These cells were further characterised by double immunofluorescence labelling and by combined immunofluorescence and enzyme cytochemical staining on frozen sections. They were labelled by the monoclonal antibodies RFD7 and anti-Leu M3 (markers of the macrophage cell line) but gave a negative reaction for the fibroblast marker IIG10 and showed no alkaline phosphatase activity. Immunoblotting detected factor XIII subunit a in macrophages isolated from placenta but not in human fibroblasts. At lower dilutions, the commercially available antibody against the b subunit of factor XIII also positively reacted with the same cell population. The facts that immunoblotting showed that the antiserum crossreacted with the a subunit and that placental macrophages did not stain strongly for the b subunit also indicate that this antigen is not present in adult connective tissue cells.
人肝脏、淋巴结和胎盘的石蜡包埋切片显示，某些结缔组织细胞的凝血因子 XIII a 亚基呈阳性。通过双重免疫荧光标记以及冷冻切片上免疫荧光与酶细胞化学染色相结合的方法，对这些细胞进行了进一步表征。它们被单克隆抗体 RFD7 和抗-Leu M3（巨噬细胞系标志物）标记，但对成纤维细胞标志物 IIG10 呈阴性反应，且未显示碱性磷酸酶活性。免疫印迹法在从胎盘中分离出的巨噬细胞中检测到了凝血因子 XIII a 亚基，但在人成纤维细胞中未检测到。在较低稀释度下，市售的抗凝血因子 XIII b 亚基抗体也与同一细胞群体呈阳性反应。免疫印迹显示抗血清与 a 亚基发生交叉反应，以及胎盘巨噬细胞对 b 亚基染色不强，这些事实也表明该抗原不存在于成人结缔组织细胞中。
