Aktaş Serdar, Sağdık Hacı Murat, Tetikoğlu Mehmet, Aktaş Hatice, Özcura Fatih, Uçar Fatma, Alışık Murat, Ergin Merve
Department of Ophthalmology, Dumlupinar University School of Medicine, Kutahya, Turkey.
Clinic of Ophthalmology, DPU Evliya Celebi Training and Research Hospital, Kutahya, Turkey.
Arq Bras Oftalmol. 2017 Jul-Aug;80(4):234-237. doi: 10.5935/0004-2749.20170057.
: We evaluated dynamic thiol/disulfide homeostasis (TDH), malondialdehyde (MDA) levels, and catalase (CAT) activity in patients with age-related macular degeneration (AMD). All analyzes were conducted on plasma samples.
: Thirty-two patients with AMD and 38 age-matched healthy controls were included. Native thiol, total thiol, and disulfide levels and TDH status were determined using a novel, automated assay. MDA levels and CAT activity were determined. Percentages were compared using the chi-squared test. The Student's t-test and Mann-Whitney U-test were used to compare quantitative variables.
: Native thiol levels were significantly lower (p=0.004) in patients with AMD (272.02 ± 52.41 µmol/l) than in healthy individuals (307.82 ± 47.18 µmol/l), whereas disulfide levels were significantly higher (p<0.001) in patients with AMD than in controls (21.64 ± 5.59 vs. 14.48 ± 5.37 µmol/L). Dynamic TDH was also significantly lower (p<0.001) in patients with AMD than in controls (13.41 ± 4.3 vs. 25.41 ± 14.52 µmol/l). No significant differences were evident in total thiol or MDA levels. Mean CAT activity was significantly higher (p=0.043) in patients with AMD compared with controls (0.035 vs. 0.018 k/ml).
: The antioxidant/oxidant balance demonstrated by dynamic TDH is shifted to the oxidative side in patients with AMD.
我们评估了年龄相关性黄斑变性（AMD）患者的动态硫醇/二硫键稳态（TDH）、丙二醛（MDA）水平和过氧化氢酶（CAT）活性。所有分析均在血浆样本上进行。
纳入32例AMD患者和38例年龄匹配的健康对照。使用一种新型自动检测方法测定天然硫醇、总硫醇和二硫键水平以及TDH状态。测定MDA水平和CAT活性。使用卡方检验比较百分比。使用学生t检验和曼-惠特尼U检验比较定量变量。
AMD患者的天然硫醇水平（272.02±52.41µmol/l）显著低于健康个体（307.82±47.18µmol/l）（p=0.004），而AMD患者的二硫键水平显著高于对照组（p<0.001）（21.64±5.59对14.48±5.37µmol/L）。AMD患者的动态TDH也显著低于对照组（p<0.001）（13.41±4.3对25.41±14.52µmol/l）。总硫醇或MDA水平无明显差异。AMD患者的平均CAT活性显著高于对照组（p=0.043）（0.035对0.018k/ml）。
AMD患者中由动态TDH所显示的抗氧化/氧化平衡向氧化方向偏移。
