Jin Long, Ko So-Ra, Lee Chang Soo, Ahn Chi-Yong, Oh Hee-Mock, Lee Hyung-Gwan
College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210-037, PR China.
Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4443-4448. doi: 10.1099/ijsem.0.002311. Epub 2017 Sep 28.
A novel Gram-negative bacterium strain, DRW22-8, was isolated from fresh water taken at a depth of 22 m at Daechung Reservoir, Republic of Korea. The cells of strain DRW22-8 were aerobic and motile with a single polar flagellum or non-motile (stalked), and formed creamy-white colonies on R2A agar. The phylogenetic analysis based on 16S rRNA gene sequencing indicated that the strain formed a separate lineage within the order Rhodobacterales, showing similarity values under 91.8 % with its closest phylogenetic neighbours, Hirschia litorea, Hirschia baltica and Hirschia maritima. The chemotaxonomic results showed Q-10 as the predominant respiratory ubiquinone, three unidentified glycolipids, an unidentified lipid and phosphatidylglycerol as the major polar lipids, and C16 : 0, 11-methyl C18 : 1, C18 : 1 ω7c and/or C18 : 1 ω6c as the major fatty acids. The DNA G+C content was 64.4 mol%. The combined genotypic and phenotypic data showed that strain DRW22-8 could be distinguished from all genera within the family Hyphomonadaceae and represented a novel genus, Asprobacter gen. nov., with the name Asprobacter aquaticus sp. nov., in the family Hyphomonadaceae. The type strain is DRW22-8 (=KCTC 42356=JCM 30469).
从韩国忠清南道大川水库22米深处采集的淡水中分离出一株新型革兰氏阴性细菌菌株DRW22-8。DRW22-8菌株的细胞为需氧型，具单极鞭毛，可运动，或不运动（有柄），在R2A琼脂上形成乳脂白色菌落。基于16S rRNA基因测序的系统发育分析表明，该菌株在红杆菌目内形成了一个独立的谱系，与其最接近的系统发育邻种——海洋希氏菌、波罗的海希氏菌和滨海希氏菌的相似性值低于91.8%。化学分类学结果显示，主要呼吸醌为Q-10，三种未鉴定的糖脂、一种未鉴定的脂质和磷脂酰甘油为主要极性脂质，主要脂肪酸为C16 : 0、11-甲基C18 : 1、C18 : 1 ω7c和/或C18 : 1 ω6c。DNA的G+C含量为64.4mol%。综合基因型和表型数据表明，DRW22-8菌株可与生丝微菌科内的所有属区分开来，代表了一个新属——厌养杆菌属，新种名为水生厌养杆菌，属于生丝微菌科。模式菌株为DRW22-8（=KCTC 42356=JCM 30469）。
