Pradenas Gonzalo A, Myers Julia N, Torres Alfredo G
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA.
Vaccines (Basel). 2017 Sep 28;5(4):33. doi: 10.3390/vaccines5040033.
is an opportunistic pathogen prevalent in cystic fibrosis patients, which is particularly difficult to treat, causing chronic and eventually fatal infections. The lack of effective treatment options makes evident the need to develop alternative therapeutic or prophylactic approaches. Vaccines, and live attenuated vaccines, are an unexplored avenue to treat infections. Here we constructed and characterized a mutant strain, which was unable to actively transport iron, to test whether this single gene deletion mutant (strain renamed GAP001) protected against an acute respiratory lethal infection. Here we show that the mutant strain GAP001 is attenuated, and effective at protecting against challenge. Intranasal administration of GAP001 to BALB/c mice resulted in almost complete survival with high degree of bacterial clearance.
是一种在囊性纤维化患者中普遍存在的机会致病菌，特别难以治疗，会引发慢性并最终致命的感染。缺乏有效的治疗选择凸显了开发替代治疗或预防方法的必要性。疫苗，尤其是减毒活疫苗，是治疗感染的一条未被探索的途径。在这里，我们构建并表征了一种无法主动转运铁的突变菌株，以测试这种单基因缺失突变体（菌株重命名为GAP001）是否能预防急性呼吸道致死性感染。在这里，我们表明突变菌株GAP001是减毒的，并且在预防攻击方面有效。向BALB/c小鼠鼻内给药GAP001导致几乎完全存活且细菌清除率很高。
