Amer Said, Helal Ibrahim B, Kamau Evelyne, Feng Yaoyu, Xiao Lihua
Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America; Department of Zoology, Faculty of Science, Kafr El sheikh University, Kafr El Sheikh, Egypt.
Department of Zoology, Faculty of Science, Tanta University, Tanta, Egypt.
PLoS One. 2015 Mar 11;10(3):e0118509. doi: 10.1371/journal.pone.0118509. eCollection 2015.
Little is known on the diversity and public health significance of Echinococcus species in livestock in Egypt. In this study, 37 individual hydatid cysts were collected from dromedary camels (n=28), sheep (n=7) and buffalos (n=2). DNA was extracted from protoscoleces/germinal layer of individual cysts and amplified by PCR targeting nuclear (actin II) and mitochondrial (COX1 and NAD1) genes. Direct sequencing of amplicons indicated the presence of Echinococcus canadenesis (G6 genotype) in 26 of 28 camel cysts, 3 of 7 sheep cysts and the 2 buffalo derived cysts. In contrast, Echinococcus granulosus sensu stricto (G1 genotype) was detected in one cyst from a camel and 4 of 7 cysts from sheep, whereas Echinococcus ortleppi (G5 genotype) was detected in one cyst from a camel. This is the first identification of E. ortleppi in Egypt.
关于埃及家畜中棘球绦虫物种的多样性及其对公共卫生的重要性，目前所知甚少。在本研究中，从单峰骆驼（n = 28）、绵羊（n = 7）和水牛（n = 2）身上收集了37个单个包虫囊肿。从单个囊肿的原头蚴/生发层中提取DNA，并通过针对核基因（肌动蛋白II）和线粒体基因（COX1和NAD1）的PCR进行扩增。扩增子的直接测序表明，28个骆驼囊肿中的26个、7个绵羊囊肿中的3个以及2个水牛囊肿中存在加拿大棘球绦虫（G6基因型）。相比之下，在1个骆驼囊肿和7个绵羊囊肿中的4个中检测到细粒棘球绦虫（G1基因型），而在1个骆驼囊肿中检测到奥氏棘球绦虫（G5基因型）。这是在埃及首次鉴定出奥氏棘球绦虫。
