Molecular Biology Laboratory, Faculty of Veterinary Medicine, University of Khartoum, Khartoum, Republic of the Sudan.
BMC Vet Res. 2013 Jun 25;9:127. doi: 10.1186/1746-6148-9-127.
Echinococcus granulosus (EG) complex, the cause of cystic echinococcosis (CE), infects humans and several other animal species worldwide and hence the disease is of public health importance. Ten genetic variants, or genotypes designated as (G1-G10), are distributed worldwide based on genetic diversity. The objective of this study was to provide some sequence data and phylogeny of EG isolates recovered from the Sudanese one-humped camel (Camelus dromedaries). Fifty samples of hydatid cysts were collected from the one- humped camels (Camelus dromedaries) at Taboul slaughter house, central Sudan. DNAs were extracted from protoscolices and/or associated germinal layers of hydatid cysts using a commercial kit. The mitochondrial NADH dehydrogenase subunit 1 (NADH1) gene and the cytochrome C oxidase subunit 1 (cox1) gene were used as targets for polymerase chain reaction (PCR) amplification. The PCR products were purified and partial sequences were generated. Sequences were further examined by sequence analysis and subsequent phylogeny to compare these sequences to those from known strains of EG circulating globally.
The identity of the PCR products were confirmed as NADH1 and cox1 nucleotide sequences using the Basic Local Alignment Search Tool (BLAST) of NCBI (National Center for Biotechnology Information, Bethesda, MD). The phylogenetic analysis showed that 98% (n = 49) of the isolates clustered with Echinococcus canadensis genotype 6 (G6), whereas only one isolate (2%) clustered with Echinococcus ortleppi (G5).
This investigation expands on the existing sequence data generated from EG isolates recovered from camel in the Sudan. The circulation of the cattle genotype (G5) in the one-humped camel is reported here for the first time.
细粒棘球蚴（EG）复合体是引起包虫病（CE）的原因，在全球范围内感染人类和其他几种动物物种，因此该病具有重要的公共卫生意义。根据遗传多样性，全球分布着 10 种遗传变异体或基因型（G1-G10）。本研究的目的是提供从苏丹单峰驼（Camelus dromedaries）中分离出的 EG 分离株的一些序列数据和系统发育。从苏丹中部塔布屠宰场的单峰驼（Camelus dromedaries）的 50 个包虫囊肿样本中采集了 DNA。使用商业试剂盒从原头蚴和/或相关的生发层中提取 DNA。线粒体 NADH 脱氢酶亚单位 1（NADH1）基因和细胞色素 C 氧化酶亚单位 1（cox1）基因被用作聚合酶链反应（PCR）扩增的靶标。PCR 产物经纯化后生成部分序列。进一步对序列进行分析和随后的系统发育分析，将这些序列与全球已知的 EG 株进行比较。
使用 NCBI（美国国立生物技术信息中心，贝塞斯达，马里兰州）的基本局部比对搜索工具（BLAST）确认 PCR 产物的身份为 NADH1 和 cox1 核苷酸序列。系统发育分析表明，98%（n=49）的分离株与加拿大棘球蚴基因型 6（G6）聚类，而只有一个分离株（2%）与棘球蚴ortleppi（G5）聚类。
本研究扩展了从苏丹骆驼中分离出的 EG 分离株产生的现有序列数据。这里首次报道了在单峰驼中循环存在牛基因型（G5）。
