Obidi Olayide, Okekunjo Foluke
Department of Microbiology, University of Lagos, Lagos, Nigeria.
World J Microbiol Biotechnol. 2017 Oct 5;33(11):196. doi: 10.1007/s11274-017-2362-y.
Microbial induced discolorations are an unsightly feature occurring on painted walls in Lagos, the commercial hub of Nigeria. Very few studies have been carried out conventionally about the microbial community structure of discolored painted walls in Nigeria therefore, knowledge of the true microbial diversity is elusive. To further our understanding of the phylogenetic diversity of representative microbial community on 40 discolored and three clean-looking buildings, a comparative DNA sequence analysis of 16S rDNA genes was undertaken. Following DNA extraction, portions of the rDNA genes were amplified by PCR and sequenced. Resulting sequences were compared with GenBank data base sequences. Fifteen unique fungal sequences and one bacterial sequence were obtained. Majority (37.50%) of rDNA sequences analyzed, represent the genus Meyerozyma of which the novel fungus Meyerozyma guilliermondii, which to our knowledge, has not yet been implicated in painted walls was detected. Clones from the discolored painted wall isolates also produced a data set in which 31.25% of sequences were related to Fusarium proliferatum and 6.25% were Pseudomonas aeruginosa. The remaining sequences clustered with members of the genera Candida (6.25%), Aspergillus (12.5%) and Cerrena (6.25%). The study provides reliable data on microbial communities on painted walls and information for paint biocide formulation.
微生物引起的变色是尼日利亚商业中心拉各斯涂漆墙壁上出现的一种难看的现象。传统上，关于尼日利亚变色涂漆墙壁微生物群落结构的研究很少，因此，对真正的微生物多样性的了解难以捉摸。为了进一步了解40座变色建筑和3座外观清洁建筑上代表性微生物群落的系统发育多样性，对16S rDNA基因进行了比较DNA序列分析。DNA提取后，通过PCR扩增rDNA基因片段并进行测序。将所得序列与GenBank数据库序列进行比较。获得了15个独特的真菌序列和1个细菌序列。分析的rDNA序列中，大多数（37.50%）代表迈耶酵母属，其中检测到新型真菌季也蒙毕赤酵母，据我们所知，它尚未与涂漆墙壁有关。来自变色涂漆墙壁分离株的克隆也产生了一个数据集，其中31.25%的序列与层出镰刀菌有关，6.25%与铜绿假单胞菌有关。其余序列与念珠菌属（6.25%）、曲霉属（12.5%）和蜡壳耳属（6.25%）的成员聚类。该研究提供了关于涂漆墙壁上微生物群落的可靠数据以及用于涂料杀菌剂配方的信息。
