Luo Xuming, Cao Jidong, Huang Junkai, Wang Zongyi, Guo Zhengyan, Chen Yihua, Ma Shumei, Liu Jun
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
DNA Res. 2018 Feb 1;25(1):25-37. doi: 10.1093/dnares/dsx035.
Frogeye leaf spot, caused by Cercospora sojina Hara, is a common disease of soybean in most soybean-growing countries of the world. In this study, we report a high-quality genome sequence of C. sojina by Single Molecule Real-Time sequencing method. The 40.8-Mb genome encodes 11,655 predicated genes, and 8,474 genes are revealed by RNA sequencing. Cercospora sojina genome contains large numbers of gene clusters that are involved in synthesis of secondary metabolites, including mycotoxins and pigments. However, much less carbohydrate-binding module protein encoding genes are identified in C. sojina genome, when compared with other phytopathogenic fungi. Bioinformatics analysis reveals that C. sojina harbours about 752 secreted proteins, and 233 of them are effectors. During early infection, the genes for metabolite biosynthesis and effectors are significantly enriched, suggesting that they may play essential roles in pathogenicity. We further identify 13 effectors that can inhibit BAX-induced cell death. Taken together, our results provide insights into the infection mechanisms of C. sojina on soybean.
由大豆尾孢菌（Cercospora sojina Hara）引起的蛙眼叶斑病是世界上大多数大豆种植国家中大豆的常见病害。在本研究中，我们通过单分子实时测序方法报道了大豆尾孢菌的高质量基因组序列。该40.8 Mb的基因组编码11,655个预测基因，通过RNA测序揭示了8,474个基因。大豆尾孢菌基因组包含大量参与次生代谢物合成的基因簇，包括霉菌毒素和色素。然而，与其他植物病原真菌相比，在大豆尾孢菌基因组中鉴定出的编码碳水化合物结合模块蛋白的基因要少得多。生物信息学分析表明，大豆尾孢菌含有约752种分泌蛋白，其中233种是效应子。在早期感染期间，代谢物生物合成和效应子的基因显著富集，表明它们可能在致病性中起重要作用。我们进一步鉴定出13种可抑制BAX诱导的细胞死亡的效应子。综上所述，我们的结果为大豆尾孢菌对大豆的感染机制提供了见解。
