Suckow Mark A, Duke Boynton Felicia D, Johnson Chad
Department of Veterinary Population Medicine, University of Minnesota;
Research Animal Resources, University of Minnesota.
J Vis Exp. 2017 Oct 2(128):53587. doi: 10.3791/53587.
Ventral abdominal hernia is a relatively common clinical condition that sometimes requires herniorraphy (surgical repair). The repair of ventral abdominal hernia typically requires implantation of a material to serve as a mechanical bridge across the defect in the abdominal wall. Biomaterials, such as porcine small intestinal submucosa (SIS), also serve as a lattice for cell growth into the implant and can naturally incorporate into the host tissue. Development of such repair materials benefits from use of animal models in which experimental abdominal wall defects are easily created and are amenable to repair in a reproducible fashion. The method offered here describes surgical creation and repair of ventral abdominal hernia in a rat model. When SIS is used to repair an experimental ventral abdominal hernia in this model, it is rapidly incorporated into host tissue within 28 days of implantation. Histologically, incorporation of their implanted material into host tissue is characterized by a robust fibrovascular response. Future refinements and applications of the rat abdominal hernia model may likely involve diabetic and/or obese animals as a means to more closely mimic common co-morbidities of man.
腹外疝是一种相对常见的临床病症，有时需要进行疝修补术（手术修复）。腹外疝的修复通常需要植入一种材料，作为跨越腹壁缺损的机械桥梁。生物材料，如猪小肠黏膜下层（SIS），也可作为细胞生长进入植入物的支架，并能自然地融入宿主组织。此类修复材料的开发得益于动物模型的使用，在这些模型中，实验性腹壁缺损易于制造，并且能够以可重复的方式进行修复。本文提供的方法描述了在大鼠模型中手术制造和修复腹外疝的过程。当在该模型中使用SIS修复实验性腹外疝时，它在植入后28天内迅速融入宿主组织。从组织学上看，植入材料融入宿主组织的特征是强烈的纤维血管反应。大鼠腹外疝模型未来的改进和应用可能会涉及糖尿病和/或肥胖动物，以此更紧密地模拟人类常见的合并症。
