Kim Jin-Hee, Chung Il Kyung, Kim Kyung-Min
School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University, Daegu, Korea.
Department of Biotechnology, Catholic University of Daegu, Gyeongsan-Si, Gyeongbuk, Korea.
PLoS One. 2017 Oct 11;12(10):e0185073. doi: 10.1371/journal.pone.0185073. eCollection 2017.
The Sweet potato, Ipomoea batatas (L.) Lam, is difficult to study in genetics and genomics because it is a hexaploid. The sweet potato study not have been performed domestically or internationally. In this study was performed to construct genetic map and quantitative trait loci (QTL) analysis. A total of 245 EST-SSR markers were developed, and the map was constructed by using 210 of those markers. The total map length was 1508.1 cM, and the mean distance between markers was 7.2 cM. Fifteen characteristics were investigated for QTLs analysis. According to those, the Four QTLs were identified, and The LOD score was 3.0. Further studies need to develop molecular markers in terms of EST-SSR markers for doing to be capable of efficient breeding. The genetic map created here using EST-SSR markers will facilitate planned breeding of sweet potato cultivars with various desirable traits.
甘薯,即Ipomoea batatas (L.) Lam,由于是六倍体,在遗传学和基因组学研究方面存在困难。国内外尚未开展甘薯研究。本研究旨在构建遗传图谱并进行数量性状位点(QTL)分析。共开发了245个EST-SSR标记,并使用其中210个标记构建了图谱。图谱总长度为1508.1厘摩,标记间平均距离为7.2厘摩。对15个特征进行了QTL分析。据此,鉴定出4个QTL,LOD值为3.0。进一步的研究需要开发EST-SSR标记方面的分子标记,以便能够进行高效育种。利用EST-SSR标记构建的遗传图谱将有助于培育具有各种优良性状的甘薯品种。