Zhou Lin, Liu Xueping, Li Xulin, He Xiangyan, Xiong Xia, Lai Jingdong
Department of Dermatology & STD, The Affiliated Hospital of Southwest Medical University, Luzhou, People's Republic of China.
Department of Dermatology & STD, Suining First People's Hospital, Suining, People's Republic of China.
Clin Cosmet Investig Dermatol. 2022 Sep 28;15:2065-2075. doi: 10.2147/CCID.S377759. eCollection 2022.
Although the changed lipid environment of the pilosebaceous unit and the growth of lipophilic () during puberty has long been considered as the trigger of acne vulgaris, the involvement of the interaction between the epidermal barrier integrity and the skin microbiome in this disease has not been fully elucidated.
The aim of this study was to analyze the differences in the epidermal barrier and skin microbiota in patients with acne vulgaris and their correlation.
The skin microbial samples and epidermal barrier data from 74 acne patients and 19 healthy subjects were collected in this cross-sectional study. The microbial diversity was analyzed based on a high-throughput sequencing approach that targets the V3-V4 region of the bacteria 16S ribosomal RNA genes.
Compared with healthy controls, acne patients had significantly increased transepidermal water loss (TEWL), pH levels, sebum, porphyrins, and red areas, and reduced skin microbiome diversity according to the goods coverage diversity index (p = 0.021), Shannon diversity index (p = 0.037), and Simpson diversity index (p = 0.023). Moreover, the diversity gradually decreased with the increase in acne grading. Based on the principal coordinate analysis (PCoA) analysis plot, the skin microbiota of acne patients and healthy controls could be divided into two different sets, which could not be used to separate acne patients with different disease severity. Finally, this study found that both TEWL and sebum were negatively associated with the Shannon and Simpson diversity index. Meanwhile, the taxa Enhydrobacter and were positively associated with TEWL, stratum corneum hydration, respectively.
This study demonstrated that acne vulgaris exists in patients with both damaged epithelial barriers and associated microbiota dysbiosis; the findings will help improve the understanding of the disease and may contribute to the development of better treatment options.
尽管长期以来，皮脂腺单位脂质环境的变化以及青春期亲脂性（）的生长一直被认为是寻常痤疮的触发因素，但表皮屏障完整性与皮肤微生物群之间的相互作用在该疾病中的作用尚未完全阐明。
本研究旨在分析寻常痤疮患者表皮屏障和皮肤微生物群的差异及其相关性。
在这项横断面研究中，收集了74例痤疮患者和19名健康受试者的皮肤微生物样本和表皮屏障数据。基于针对细菌16S核糖体RNA基因V3-V4区域的高通量测序方法分析微生物多样性。
与健康对照组相比，根据商品覆盖多样性指数（p = 0.021）、香农多样性指数（p = 0.037）和辛普森多样性指数（p = 0.023），痤疮患者的经表皮水分流失（TEWL）、pH值、皮脂、卟啉和红色区域显著增加，皮肤微生物群多样性降低。此外，随着痤疮分级的增加，多样性逐渐降低。基于主坐标分析（PCoA）分析图，痤疮患者和健康对照组的皮肤微生物群可分为两组，这两组不能用于区分不同疾病严重程度的痤疮患者。最后，本研究发现TEWL和皮脂均与香农和辛普森多样性指数呈负相关。同时，水生杆菌属和分别与TEWL、角质层水合呈正相关。
本研究表明，寻常痤疮患者存在上皮屏障受损和相关微生物群失调；这些发现将有助于提高对该疾病的认识，并可能有助于开发更好的治疗方案。
