English Daniel Fine, McKenzie Sam, Evans Talfan, Kim Kanghwan, Yoon Euisik, Buzsáki György
Neuroscience Institute, New York University, New York, NY 10016, US.
University of Michigan, Ann Arbor, MI 48109, US.
Neuron. 2017 Oct 11;96(2):505-520.e7. doi: 10.1016/j.neuron.2017.09.033.
Excitatory control of inhibitory neurons is poorly understood due to the difficulty of studying synaptic connectivity in vivo. We inferred such connectivity through analysis of spike timing and validated this inference using juxtacellular and optogenetic control of presynaptic spikes in behaving mice. We observed that neighboring CA1 neurons had stronger connections and that superficial pyramidal cells projected more to deep interneurons. Connection probability and strength were skewed, with a minority of highly connected hubs. Divergent presynaptic connections led to synchrony between interneurons. Synchrony of convergent presynaptic inputs boosted postsynaptic drive. Presynaptic firing frequency was read out by postsynaptic neurons through short-term depression and facilitation, with individual pyramidal cells and interneurons displaying a diversity of spike transmission filters. Additionally, spike transmission was strongly modulated by prior spike timing of the postsynaptic cell. These results bridge anatomical structure with physiological function.
由于在体内研究突触连接存在困难，兴奋性神经元对抑制性神经元的控制机制目前仍知之甚少。我们通过分析峰电位时间来推断这种连接性，并利用行为小鼠中突触前峰电位的近细胞和光遗传学控制来验证这一推断。我们观察到，相邻的CA1神经元之间连接更强，且浅层锥体细胞向深层中间神经元的投射更多。连接概率和强度存在偏差，少数高度连接的枢纽细胞。发散的突触前连接导致中间神经元之间的同步。汇聚的突触前输入的同步增强了突触后驱动。突触后神经元通过短期抑制和易化来读取突触前放电频率，单个锥体细胞和中间神经元表现出多样的峰电位传递滤波器。此外，峰电位传递受到突触后细胞先前峰电位时间的强烈调制。这些结果将解剖结构与生理功能联系起来。
