Immunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR "Demokritos", 15310 Aghia Paraskevi, Greece.
ThetaMetrisis S.A., 12243 Egaleo, Greece.
Biosensors (Basel). 2017 Oct 13;7(4):46. doi: 10.3390/bios7040046.
The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low molecular weight analytes (e.g., pesticides), is described. At the same time, the passage from single to multi-analyte determinations, and from a laboratory prototype set-up to a compact device appropriate for on-site determination, is outlined. The improvements made on both the sensor and the optical set-up, and the concomitant advances in the analytical characteristics and the robustness of the assays performed with the different layouts, are also presented. Finally, the future perspectives of the system, aiming for the creation of a standalone instrument to be used by non-experts, will be discussed.
本文介绍了一种基于白光反射光谱（WLRS）的传感平台的开发。该系统的发展历程包括聚合物薄膜特性表征和挥发性有机化合物的传感，以及用于无标记测定高分子量（如蛋白质）或低分子量分析物（如农药）的生物传感器。同时，还描述了从单分析物测定到多分析物测定，以及从实验室原型装置到适用于现场测定的紧凑型装置的转变。本文还介绍了传感器和光学装置的改进，以及不同设计的分析特性和分析物测定稳健性的提高。最后，还讨论了该系统的未来展望，旨在开发一种可供非专业人员使用的独立仪器。
