Pawar Anil T, Vyawahare Niraj S
Centre for Research and Development, PRIST University, Thanjavur 613403, India.
Department of Pharmacology, MAEER's Maharashtra Institute of Pharmacy, Kothrud, Pune 411038, India.
J Tradit Complement Med. 2017 Jan 17;7(4):476-486. doi: 10.1016/j.jtcme.2016.12.012. eCollection 2017 Oct.
The methanolic whole plant extract of (gǎnyìng cǎo) has been found to possess antiurolithiatic effect. The present study was undertaken to evaluate the antiurolithiatic effect of some fractions of methanolic whole plant extract of (MBS) in rats as a step toward activity-directed isolation of antiurolithiatic component. The MBS was successively extracted with dichloromethane, ethyl acetate, ethanol and water to obtain fractions. Sodium oxalate (70 mg/kg, i.p.) was administered to rats for seven days to develop calcium oxalate urolithiasis. These rats were treated with two doses (20 and 40 mg/kg, p.o.) of the fractions, 1 h before sodium oxalate injections. Antiurolithiatic activity was assessed by estimating biochemical changes in urine, serum and kidney homogenate along with histological changes in kidney tissue. Sodium oxalate administration caused biochemical alterations in urine which was found to be prevented significantly by the ethyl acetate fraction. Supplementation with ethyl acetate fraction prevented the elevation of serum creatinine, uric acid and blood urea nitrogen levels. The elevated calcium, oxalate and phosphate levels in the kidney tissue homogenate of lithiatic rats were significantly reduced by the treatment with ethyl acetate fraction. The ethyl acetate fraction also caused significant decrease in lipid peroxidation activity, accumulation of calcium oxalate deposits and histological changes in the kidney tissue. The results showed that the antiurolithiatic component of the methanolic whole plant extract of the plant is contained in the ethyl acetate fraction. The effect is attributed to its diuretic, antioxidant, nephroprotective properties and effect on lowering the concentration of urinary stone-forming constituents.
已发现（感应草）的甲醇全株提取物具有抗尿路结石作用。本研究旨在评估感应草甲醇全株提取物（MBS）的某些馏分对大鼠的抗尿路结石作用，作为朝着抗尿路结石成分活性导向分离迈出的一步。将MBS依次用二氯甲烷、乙酸乙酯、乙醇和水萃取以获得馏分。给大鼠腹腔注射草酸钠（70mg/kg），持续7天以诱发草酸钙尿路结石。在注射草酸钠前1小时，用两剂（20和40mg/kg，口服）馏分对这些大鼠进行治疗。通过评估尿液、血清和肾匀浆中的生化变化以及肾组织的组织学变化来评估抗尿路结石活性。草酸钠给药导致尿液中的生化改变，而乙酸乙酯馏分可显著预防这种改变。补充乙酸乙酯馏分可预防血清肌酐、尿酸和血尿素氮水平的升高。用乙酸乙酯馏分治疗可显著降低结石大鼠肾组织匀浆中升高的钙、草酸盐和磷酸盐水平。乙酸乙酯馏分还可显著降低脂质过氧化活性、草酸钙沉积物的积累以及肾组织的组织学变化。结果表明，该植物甲醇全株提取物的抗尿路结石成分存在于乙酸乙酯馏分中。其作用归因于其利尿、抗氧化、肾保护特性以及对降低尿路结石形成成分浓度的作用。
