Department of Viral infection and International Health, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan.
National Hospital of Pediatrics, Hanoi 100000, Vietnam.
Int J Mol Sci. 2017 Oct 19;18(10):2185. doi: 10.3390/ijms18102185.
Here, we investigated the effects of the probiotic strain Shirota (LcS) on immune profiles and intestinal microbial translocation among children infected with human immunodeficiency virus (HIV). This prospective study included 60 HIV-infected children-including 31 without antiretroviral therapy (ART) (HIV(+)) and 29 who received ART for a median of 3.5 years (ART(+)) and 20 children without HIV infection (HIV(-)). Participants were recruited in Vietnam. All children were given fermented milk containing LcS (6.5 × 10⁸ cfu) daily for 8 weeks. Before and after LcS ingestion, blood samples were collected for virological, immunological, and bacteriological analyses. After LcS ingestion, peripheral CD4⁺ T-cell and Th2 (CXCR3CCR6CD4⁺) counts significantly increased in both HIV-infected groups; Th17 (CXCR3CCR6⁺CD4⁺) counts increased in all three groups; regulatory T-cell (CD25CD4⁺) counts decreased in the ART(+) and HIV(-) groups; activated CD8⁺ cells (CD38⁺HLA-DR⁺CD8⁺) decreased from 27.5% to 13.2% ( < 0.001) in HIV(+) children; and plasma HIV load decreased slightly but significantly among HIV(+) children. No group showed a significantly altered frequency of bacterial 16S/23S rRNA gene detection in the plasma. No serious adverse events occurred. These findings suggest that short-term LcS ingestion is a safe supportive approach with immunological and virological benefits in HIV-infected children.
在这里，我们研究了益生菌菌株 Shirota（LcS）对感染人类免疫缺陷病毒（HIV）的儿童免疫谱和肠道微生物易位的影响。这项前瞻性研究包括 60 名感染 HIV 的儿童，包括 31 名未接受抗逆转录病毒治疗（ART）的儿童（HIV(+））和 29 名接受 ART 治疗中位数为 3.5 年的儿童（ART(+））和 20 名未感染 HIV 的儿童（HIV(-））。参与者在越南招募。所有儿童均每日服用含有 LcS（6.5×10⁸ cfu）的发酵乳，持续 8 周。在摄入 LcS 前后，采集血液样本进行病毒学、免疫学和细菌学分析。摄入 LcS 后，两组 HIV 感染儿童外周血 CD4⁺T 细胞和 Th2（CXCR3CCR6CD4⁺）计数均显著增加；三组 Th17（CXCR3CCR6⁺CD4⁺）计数均增加；ART(+)和 HIV(-)组调节性 T 细胞（CD25CD4⁺）计数减少；CD38⁺HLA-DR⁺CD8⁺活化的 CD8⁺细胞从 HIV(+)儿童的 27.5%降至 13.2%（<0.001）；HIV(+)儿童血浆 HIV 载量略有但显著下降。各组血浆中细菌 16S/23S rRNA 基因检测的频率均无明显变化。未发生严重不良事件。这些发现表明，短期摄入 LcS 是一种安全的支持方法，可改善 HIV 感染儿童的免疫和病毒学状况。
