Boroumand Moghaddam Amin, Moniri Mona, Azizi Susan, Abdul Rahim Raha, Bin Ariff Arbakariya, Navaderi Mohammad, Mohamad Rosfarizan
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Young Research and Elite Club, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.
Genes (Basel). 2017 Oct 20;8(10):281. doi: 10.3390/genes8100281.
Green products have strong potential in the discovery and development of unique drugs. Zinc oxide nanoparticles (ZnO NPs) have been observed to have powerful cytotoxicity against cells that cause breast cancer. The present study aims to examine the cell cycle profile, status of cell death, and pathways of apoptosis in breast cancer cells (MCF-7) treated with biosynthesized ZnO NPs. The anti-proliferative activity of ZnO NPs was determined using MTT assay. Cell cycle analysis and the mode of cell death were evaluated using a flow cytometry instrument. Quantitative real-time-PCR (qRT-PCR) was employed to investigate the expression of apoptosis in MCF-7 cells. ZnO NPs were cytotoxic to the MCF-7 cells in a dose-dependent manner. The 50% growth inhibition concentration (IC) of ZnO NPs at 24 h was 121 µg/mL. Cell cycle analysis revealed that ZnO NPs induced sub-G₁ phase (apoptosis), with values of 1.87% at 0 μg/mL (control), 71.49% at IC, 98.91% at IC, and 99.44% at IC. Annexin V/propidium iodide (PI) flow cytometry analysis confirmed that ZnO NPs induce apoptosis in MCF-7 cells. The pro-apoptotic genes , , , and were upregulated, whereas anti-apoptotic genes , , and were downregulated in a dose-dependent manner. The arrest and apoptosis of MCF-7 cells were induced by ZnO NPs through several signalling pathways.
绿色产品在独特药物的发现和开发方面具有巨大潜力。已观察到氧化锌纳米颗粒（ZnO NPs）对引发乳腺癌的细胞具有强大的细胞毒性。本研究旨在检测经生物合成的ZnO NPs处理的乳腺癌细胞（MCF - 7）的细胞周期概况、细胞死亡状态及凋亡途径。使用MTT法测定ZnO NPs的抗增殖活性。使用流式细胞仪评估细胞周期分析和细胞死亡模式。采用定量实时聚合酶链反应（qRT - PCR）研究MCF - 7细胞中凋亡的表达。ZnO NPs对MCF - 7细胞具有剂量依赖性细胞毒性。ZnO NPs在24小时时的50%生长抑制浓度（IC）为121μg/mL。细胞周期分析显示，ZnO NPs诱导亚G₁期（凋亡），在0μg/mL（对照）时为1.87%，在IC时为71.49%，在IC时为98.91%，在IC时为99.44%。膜联蛋白V/碘化丙啶（PI）流式细胞术分析证实ZnO NPs诱导MCF - 7细胞凋亡。促凋亡基因、、、和被上调，而抗凋亡基因、和以剂量依赖性方式下调。ZnO NPs通过多种信号通路诱导MCF - 7细胞停滞和凋亡。
