Moghaddam Amin Boroumand, Moniri Mona, Azizi Susan, Rahim Raha Abdul, Ariff Arbakariya Bin, Saad Wan Zuhainis, Namvar Farideh, Navaderi Mohammad
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Young Research and Elite Club, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.
Molecules. 2017 May 24;22(6):872. doi: 10.3390/molecules22060872.
The potential ability of a new yeast strain, , in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD and TEM results, the ZnO-NPs with a hexagonal wurtzite structure and a particle crystal size of ~10-61 nm was formed at different reaction times. Combing XRD, TEM, and PL results, it was revealed that the sample prepared at intermediate duration (24 h) has the most favorable nanosized structure with the lowest defect concentration. The biomedical properties of ZnO-NPs as free radical scavenging activity, cytotoxicity and antibacterial agents were characterized. Biosynthesized ZnO-NPs showed strong DPPH free radical scavenging and a dose dependent toxicity with non-toxic effects on Vero cells for concentrations below 190 µg/mL. Desirable bactericidal activity was shown by the ZnO-NPs on Gram-positive bacteria (, and and Gram-negative bacteria ( and ). A maximum inhibition zone of ~19 mm was observed for at a concentration of 100 µg/mL for sample prepared at 24 h The results from this study reveal that ZnO-NPs possesses potential for many medical and industrial applications.
本研究探索了一种新型酵母菌株通过绿色方法合成氧化锌纳米颗粒（ZnO-NPs）的潜在能力。研究了反应时间（12、24和36小时）对所得ZnO纳米颗粒结构的影响。从XRD和TEM结果来看，在不同反应时间形成了具有六方纤锌矿结构且颗粒晶体尺寸约为10 - 61nm的ZnO-NPs。综合XRD、TEM和PL结果表明，在中间时长（24小时）制备的样品具有最有利的纳米结构，缺陷浓度最低。对ZnO-NPs的生物医学特性进行了表征，包括自由基清除活性、细胞毒性和抗菌剂特性。生物合成的ZnO-NPs表现出较强的DPPH自由基清除能力，以及对浓度低于190μg/mL的Vero细胞无毒性作用的剂量依赖性毒性。ZnO-NPs对革兰氏阳性菌（、和）和革兰氏阴性菌（和）表现出良好的杀菌活性。对于在24小时制备的样品，在浓度为100μg/mL时，观察到对的最大抑制圈约为19mm。本研究结果表明，ZnO-NPs在许多医学和工业应用中具有潜力。
