Quan Jianping, Ding Rongrong, Wang Xingwang, Yang Ming, Yang Yang, Zheng Enqin, Gu Ting, Cai Gengyuan, Wu Zhenfang, Liu Dewu, Yang Jie
College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
National Engineering Research Center for Breeding Swine Industry, Guangdong Wens Foodstuffs Co., Ltd, Yunfu 527400, China.
Asian-Australas J Anim Sci. 2018 Apr;31(4):480-488. doi: 10.5713/ajas.17.0356. Epub 2017 Oct 20.
Average daily gain (ADG) is an important target trait of pig breeding programs. We aimed to identify single nucleotide polymorphisms (SNPs) and genomic regions that are associated with ADG in the Duroc pig population.
We performed a genome-wide association study involving 390 Duroc boars and by using the PorcineSNP60K Beadchip and two linear models.
After quality control, we detected 3,5971 SNPs, which included seven SNPs that are significantly associated with the ADG of pigs. We identified six quantitative trait loci (QTL) regions for ADG. These QTLs included four previously reported QTLs on chromosome (SSC) 1, SSC5, SSC9, and SSC13, as well as two novel QTLs on SSC6 and SSC16. In addition, we selected six candidate genes (general transcription factor 3C polypeptide 5, high mobility group AT-hook 2, nicotinamide phosphoribosyltransferase, oligodendrocyte transcription factor 1, pleckstrin homology and RhoGEF domain containing G4B, and ) associated with ADG on the basis of their physiological roles and positional information. These candidate genes are involved in skeletal muscle cell differentiation, diet-induced obesity, and nervous system development.
This study contributes to the identification of the casual mutation that underlies QTLs associated with ADG and to future pig breeding programs based on marker-assisted selection. Further studies are needed to elucidate the role of the identified candidate genes in the physiological processes involved in ADG regulation.
平均日增重（ADG）是猪育种计划的一个重要目标性状。我们旨在鉴定与杜洛克猪群体中ADG相关的单核苷酸多态性（SNP）和基因组区域。
我们对390头杜洛克公猪进行了全基因组关联研究，并使用了猪60K SNP芯片和两个线性模型。
经过质量控制后，我们检测到35971个SNP，其中包括7个与猪ADG显著相关的SNP。我们鉴定出6个ADG的数量性状位点（QTL）区域。这些QTL包括先前在1号染色体（SSC1）、SSC5、SSC9和SSC13上报道的4个QTL，以及在SSC6和SSC16上的2个新QTL。此外，我们根据其生理作用和位置信息选择了6个与ADG相关的候选基因（通用转录因子3C多肽5、高迁移率族AT钩2、烟酰胺磷酸核糖基转移酶、少突胶质细胞转录因子1、含pleckstrin同源和RhoGEF结构域的G4B以及）。这些候选基因参与骨骼肌细胞分化、饮食诱导的肥胖和神经系统发育。
本研究有助于鉴定与ADG相关的QTL潜在的偶然突变，并为基于标记辅助选择的未来猪育种计划做出贡献。需要进一步研究以阐明所鉴定的候选基因在ADG调节所涉及的生理过程中的作用。
